Sahlgrenska Academy at University of GothenburgDepartment of Internal Medicine and Clinical Nutrition3AbstractTitle: How does isoflavones in combination with soy protein impact cholesterol levels in hypercholesterolemic postmenopausal women relative to milk protein?Author: Emma Edberg och Emma NilssonSupervisor: Lena HulthénExaminer: Anna WinkvistProgramme: Dietician stydy programme,180/240 hpType of paper: Examination paper, 15 hpDate: 2014-05-26Background: Hypercholesterolemia is a major risk factor for cardiovascular disease and the number one cause of death among women in Western countries. Declining levels of estrogen at menopause could be a contribution factor. Isoflavones is a vegetable substance, with structural and functional similarities to the human estrogen. This is a potential reason for possible cholesterol-lowering effects.

The coproduct, dried distillers grains + solubles (DDGS) from ethanol production based on wheat contains much rumen degradable protein and fiber with low digestibility. To compliment this quality in an effective way a grass silage low in protein and much digestible fiber is needed. The aim of the study was to investigate the effects of adjusting the crude-protein concentration and fiber quality of the silage when feeding DDGS and of varying the inclusion level of DDGS, when feeding the adjusted grass silage, on intake, milk yield and composition, faecal traits and feed efficiency. Four different total mixed rations were compared. The normal grass-clover silage had a crude protein content of 17,8 % and an NDF content of 46,2 % of dry matter. The adapted grass silage had a crude protein content of 14,6 % and an NDF content of 52,9 % of dry matter. The diets were formulated to have similar nutritional content except for A + 4 which had a higher content of rumen degradable crude protein and a lower concentration of NDF.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

Background: The majority of nurses experience work relatedstress, which can lead to a negative impact on patient care. Thestress can be handled by coping strategies. Aim: To illuminatecoping strategies among nurses experiencing work relatedstress. Method: The study has been accomplished as aliterature study, with a systematic review of scientific articles.Findings: Following coping strategies appeared: problemsolving, long-term strategies, passive strategies, leisurestrategies, social support and mental strategies. Conclusion:Awareness of coping strategies for dealing with work relatedstress, can lead to improvement in patient care..

The purpose of this thesis was to look at the relationship between the experiences of work related stress among male bank employees and how they dealt with potential stress. The thesis focused on male bank employee?s experiences and management of work related stress with the control requirements and support model as a starting point. Work related stress is a phenomenon that has become a serious problem with economic impact for both the individual and organizations. Increased absence from work due to medical issues is one example of such consequences.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

This essay focus on work related stress and psychosocial enviroment in working life. The purpose of this study is to present a detailed description of regulation of the the Occupational Safety and Health Act and investigate how well employees are being protected by the law.I will also explain with the help of statistic which employees that most often suffer from work related stress and the results is discussed from a gender perspective.Work related phycosocial health is a big issue in todays workplaces, and people who suffers from stress are supposed to be protecteded by the the Occupational Safety and Health Act, but is that really the truth? The Occupational Safety and Health Act is a frame law, meaning the law is general and needs binding regulations to define the rules, and there is no such act about work related stress at the moment. No employer has ever been convicted for work related stress. At the same time the Occupational Safety and Health Act explains that the employer has a responsibility to take arrangement to prevent mental illness in working life.It?s mostly women who suffer from work related stress.

Aspen (Populus tremula L.) and goat willow (Salix caprea L.) are two important tree species in the aspect of nature conservation. Due to the elimination of forest fires, a former negative attitude towards deciduous trees in forestry and a strong herbivore pressure, these tree species have been negatively affected during the last fifty years. The aim of this study was to investigate whether the occurrence of aspen and goat willow could be related to either human impact or to site properties, such as ground moisture and vegetation type. A side study included an inventory of a number of cyanobacterial tree lichens on aspen and goat willow in order to try to relate the occurrence of these lichens species to stand history and stand properties. The study was performed in Hamra State Forest in northern Dalarna. Nature reserves were compared with stands in managed areas.

The urbanization of the world is increasing. How it affects bird populations have been well studied during the last decade, and comparisons between urban and non-urban habitats have been carried out. A common method for measuring habitat quality is to calculate the population density. This method is easy to use, but have been criticized during the last couple of years. In this study, data of population densities was compared with data on reproduction success.

The aim for the study was to examine a harwarder´s (combined harvester-forwarder) productivity when harvesting forest fuel in first thinning stands that contains Lodgepole pine and calculate the economy of the harvesting work and estimate the quality of the thinning . The field study area where located near Arvån, about 15 kilometres south ?east from the city of Lycksele. The field studies were taken place in three different stands. All tree stands had the same tree age, but the tree species combination and the density varied.

To better understand how various factors affect the establishment of forest and trees around the tree line-ecotone, this study was conducted in Troms County, Norway. The altitude of different forest and tree limits together with the surrounding vegetation type were collected during August 2011. Comparisons of the collected data were made with previously collected data from the same sites conducted 1914 and 1915. On average the forest limit has expanded with 26 meters in altitude, however, an expansion is not statistically significant for the whole area. The tree limit has significantly expanded in altitude.

Prescribed burning was commonly used for site preparation in Sweden to establish new forests until the 1960?s, when mechanical scarification was introduced. During recent decades the interest in prescribed burnings has increased again, mainly due to certifications of forestry stating that 5% of the regeneration areas should be burned on dry and mesic soils. The objective of the study was to evaluate actual influence of prescribed burning compared to other site preparation on stand stem density, growth parameters and tree damages for Scots pine (Pinus sylvestris L.) after direct seeding and planting. The study also attempts to simulate growth until first thinning.

Arbetet beskriver en analys av innehållet av membranbundna och periplasmiska c-cytokromer hos perkloratodlade GR-1 och jämförelser med c-cytokrominnehållet hos Ideonella dechloratans och andra kända c-cytokromer, samt med genomet för Dechloromonas aromatica. Den jämförande studien av c-cytokromer gjordes med syftet att undersöka en hypotes om att bakterierna använder olika vägar för elektronöverföring till det periplasmiska enzymet (per)kloratreduktas. Cellmembran från GR-1 renframställdes genom ultracentrifugering och periplasma preparerades fram med hjälp av osmotisk chock. Fraktionerna analyserades sedan med SDS-PAGE och peptider med kovalent bundet hem (c-cytokromer) detekterades med hjälp av en specifik färgreaktion. Även Touchdown PCR med degenererade primrar genomfördes på isolerat DNA från GR-1 i ett försök att finna en gen kodande för ett NapC/NirT-liknande protein.

Bovin spongiform encephalopathy (BSE) är en sjukdom som drabbar nötkreatur, och varssmittämne är det ofullständigt utforskade prionproteinet. Detta protein finns i vår egen kropp,men då veckat till strukturen av en alfahelix istället för ett betaflak. När vi får i oss ettfelveckat protein verkar det som att en domino-effekt sker och alla normala proteiner avdenna sort veckar sig fel och ansamlas i centrala nervsystemet (CNS). Människor som ätersmittat kött kan drabbas av den humana formen, variant Creutzfeldt-Jakobs sjukdom (vCJD),som är en neurodegenerativ sjukdom med lång inkubationstid och som oavkortat leder tilldöden. I denna litteraturstudie görs ett försök att belysa dessa sjukdomar och problematikenrunt omkring, med betoning på BSE.