Sök:

Sökresultat:

159 Uppsatser om Isolation - Sida 3 av 11

Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous Isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous Isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous Isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous Isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

Ungdomars upplevelser av att leva med cancer

Cancer is one of the most common diseases and it affects not only adults but also many adolecents and children every year. Those who are affected of disease face many challenges that will affect their lives. The aim of this literature review was to illuminate adolecents experience of cancer and how it affects them in their daily living. The result showed that the adolecents experience many physical and mental side-effects from disease and treatment. They expressed the need of information, support and understanding from their families, friends, school and care givers.

Kvinnors känsla av sammanhang och upplevelsen av bröstcancer

Cancer is one of the most common diseases and it affects not only adults but also many adolecents and children every year. Those who are affected of disease face many challenges that will affect their lives. The aim of this literature review was to illuminate adolecents experience of cancer and how it affects them in their daily living. The result showed that the adolecents experience many physical and mental side-effects from disease and treatment. They expressed the need of information, support and understanding from their families, friends, school and care givers.

Utvärdering av olika diagnostiska metoder för infektioner med bovint coronavirus hos nötkreatur :

This paper shortly describes the coronavirus family, bovine corona viruses (BCV) properties and two diseases that BCV causes, winter dysentery and calf diarrhoea. The purpose of the study was to compare different diagnostic methods to detect BCV. Different methods are discussed, PCR, ELISA, immunofluorescense and virus Isolation. Investigations were made in three different herds with winter dysentery; one dairy cattle farm with about 100 cows of different ages, one testing station for bulls with about 150 bulls and another small dairy cattle farm with 26 cows and 20 replacement heifers and calves. Faeces and nasal swabs were analysed with PCR and ELISA. The result shows that PCR seems to be a reliable method for detecting BCV but that the ELISA test can not be used as a reliable diagnostic method to analyse samples from animals with winter dysentery..

Lactobacilli in the gastrointestinal tract of dog and wolf : isolation, identification and characterization of L. reuteri

Since the first wolf was taken as a companion animal a lot have happened with the biology of these animals. Is it possible that changes in the gut microbiota of the domestic dog are involved in the increasing numbers of animals with gastrointestinal disorders? Probiotics have been proven to promote the immune system of dogs by increasing concentrations of neutrophils and monocytes, decreasing the fragility of erythrocytes and increasing the serum IgG concentrations. Probiotics have also been shown to improve the fecal consistency, fecal dry matter and defecation frequency of dogs. The aim of this study was to find out if the bacterial counts in the gut of dogs differed from the gut of wolves, with the focus on lactobacilli.

Patienters upplevelser av att leva med fibromyalgi

Fibromyalgia is a chronic pain disorder. It affects muscles and connective tissue. It's a very complex disorder that has no adequate treatment or cure. The research has progressed but there are still some questions to be answered. Quality of life for these patients is decreased and leads to suffering.

Att vara förälder til barn med astma : En litteraturstudie

Fibromyalgia is a chronic pain disorder. It affects muscles and connective tissue. It's a very complex disorder that has no adequate treatment or cure. The research has progressed but there are still some questions to be answered. Quality of life for these patients is decreased and leads to suffering.

Individens upplevelse av att leva med självskadande beteende : En retrospektiv litteraturstudie

Self-harm is common among young people in Sweden. Therefore people who work with these young people need to have knowledge about self-harm to be able to provide the right help. The aim of this study was to investigate how the individual experience self-harm, and how this could be understood. The empirical material has been based on consisted of 28 life stories. 24 of them were written by women and 4 by men.

Omsorg i nöd och lust : kvinnliga och manliga anhörigvårdares upplevelser av att vårda sina makar

In using a qualitative method have I interviewed six women and six men taking care of their wives respectively husbands suffering from physical disease, disfunction or demental disease. The purpose was to find out whether male and female caretakers were differently influenced in their situation, relationship and in their partners, when they became caretakers of their spouse, depending on what the partner suffered from.The results showed that, when a husband or wife was hit by disease or disfunction, the caretaker was influenced both in the partners situation, the relationship and the role towards the partner. The couple still lived in a close relationship and could communicate. For the carers spouse suffered from a demental disease, there was no communication as before or possibility to remember. The carers also felt the loss of community, nearness and sympathy.

Att börja från noll. En studie i storstadssatsningens försök att öka det politiska deltagandet hos Malmös invandrare

This thesis title starting from zero alludes to storstadssatsningen's democratic work being accomplished among immigrants in Malmö. My thesis examines which types of political participation storstadssatsningen were trying to increase. The goal was to turn the immigrants into political participants, but in many of the projects the democratic work had to focus on building a ground for democratic participation by bringing Isolation to an end and teaching the Swedish language. This may have been the reason that there were not extensive forms of resources to put into projects actually raising the political awareness. Some of the projects can however be said to increase a very large number of types of political participation, both temporary and every day participation.

Gröna tak som del i ett hållbart stadsbyggande

Green roofs have seen a revival in the society of today. They provide many functions of which both society as a whole and the individual can take part. In particular extensive green roofs, that requires little or even zero maintenance, provides very valuable functions. Green roofs affect our environment and our economy in a positive manner as well as the physical expression of buildings. In this bachelor thesis the impact of green roofs on society has been examined throughout literature studies. The result as a compilation of contemporary research in this field is presented together with a discussion about the use of green roofs in general. The two major arguments for an extended use of green roofs are, the high stormwater retention capacity and the cost-benefits seen over time compared to a conventional roof.

Att köpa en identitet : en etnografisk studie i en mobilbutik

Purpose/Aim: The purpose of this thesis is to study the interaction between seller and buyer in a mobile phone store. The specific research questions under investigation are:Are there any underlying factors behind mobile purchase, and if so, what are they? Are mobile purchases driven by lifestyle factors and do customers try and create identities when purchasing new mobile phones?Material/Method: The study employs an ethnographic method when trying to answer the posed research questions. I have under a period of two weeks studied the seller-buyer interaction in a mobile phone store. In order to further increase the accuracy of my findings, I have complemented my observations with a sample of buyer and seller interviews.Main results: The observations and interviews were initially studied in Isolation, resulting in different themes.

Magnetiska egenskaper hos Öje-Diabasen, Dalecarlia

A study of paleomagnetic remanence and anisotropy of magnetic susceptibility (AMS) has been performed on the Öje-Diabase in Dalarna, Sweden, and on basic dikes associated with the alkaliquartzsyenite/granite Noran-pluton in central Sweden. Based on Isolation of directions of characteristic magnetization in the basic rocks, a division into three different groups (A,B,C) has been made. Group Aconsists of dolerites of Jotnian origin, both dolerites from the Öje-Diabase and dikes associated with the Noran-pluton are included in this group, making them coeval. Group B is suspected to show a re-magnetization from the later part of the Svekonorwegian orogeny. Group C belongs to the Blekinge-Dalarna-Dolerites (BDD). Thermal demagnetization of dolerites revealed two different types of demagnetization behaviours, one where magnetite clearly was the source of the remanence, and an other with a linear decrease in magnetization intensity. The measurements of magnetic anisotropy reveals a magnetic fabric with an almost horizontal foliation and a lineation that indicates a fairly uniform NNW/ S-SE flow direction of the magma..

Support by a nurse to parents with a colic infant

Every six baby that is born gets colic. It means that the child cries for at least three hours three days a week. For the child?s parents this is one big strain and feelings like despair, helplessness and Isolation are common. The nurse?s task is to support the parents through respectful and empathic actions in order to relieve sufferings and increase their feelings of confidence.

Myndigheten och den ensamstående föräldern. En studie av ensamstående föräldrar med barn och deras behov

In this study we describe the needs of lone parents and how the authorities fulfill these needs. The needs contain both economic and social aspects, such as the access to an employment, childcare and the possibility to social activities. For the lone parents being able to work the childcare provision must improve and be available in a greater extent during non-traditional working hours. It is also required that the housing benefit ceiling is increased so that the parents may work more hours without risking the loss of the housing benefit. The statistic about the housing benefit which we present shows that many of those with a low income are lone parents.

<- Föregående sida 3 Nästa sida ->