Inflammatory Bowel Disease hos häst är ett allvarligt tillstånd som kan ta olika uttryck, gemensamt är dock en inflammation i tarmen med svåra skador som följd. Olika etiologier har föreslagits, men det är troligt att immunsystemet har en avgörande roll i sjukdomsutvecklingen. I den normala tarmen utvecklar immunförsvaret tolerans mot kommensal mikroflora. En viktig pusselbit i den utvecklingen är aktiveringen av naiva T-celler till regulatoriska T-celler som dämpar immunförsvaret. De regulatoriska T-cellerna verkar suppressivt på T-hjälparceller via cytokiner och cellcellkontakt samt kan aktiveras centralt i thymus eller perifert, exempelvis i tarmen.

In this study, 29 calves were dehorned by heat cauterisation at the age of 1 to 8 weeks. Before the procedure, the calves were given local anaesthetic but were not sedated. In connection with the dehorning, 14 calves were given the non-steroidal anti-Inflammatory drug (NSAID) meloxicam and 15 calves were given placebo. During the following three days behavioural studies were performed to investigate if the different treatments had any effect on the calves´ postoperative behaviour. Head shakes, head rubs, kicks and ear flicks were studied, as earlier studies have shown that an increase in these behavioural patterns suggests postoperative pain.

The purpose of this project was to investigate if retention of polar compounds that are given to treat tuberculosis, diabetes, Inflammatory bowel disease and childhood leukemia could be obtained with HILIC separation. By varying different parameters for different types of columns the compounds were analyzed with the aim of finding guidelines for future method optimizations. To perform these analyzes three different columns were tested ? ZIC-HILIC (silica-based with zwitterions), ZIC-pHILIC (polymer-based with zwitterions) and XBridge Amide (amide functions). The results were evaluated with selected quality measures.

Träningsinducerad lungblödning, Exercise-induced pulmonary hemorrhage (EIPH), är ett stort problem hos travare och galoppörer i hela världen. Det får anses klarlagt att mekanismen bakom EIPH är så kallad stress-failure, utmattningsskador, i blod-gasbarriären vilket beror på att hästarna har en respiration och cirkulation som ger extrema påfrestningar på denna sköra struktur. En bidragande faktor till stress-failure tycks kunna vara att hypertension i lungvenerna leder till att dessa remodelleras och förtjockas, vilket i sin tur leder till ännu högre tryck i lungkapillärerna. Den här litteraturstudien handlar om hur inflammation i lungorna kan ge upphov till EIPH. Inflammation finns med i problematiken runt EIPH både som en bakomliggande faktor och som ett resultat av blödningen.

Thoroughbred racehorses have suffered from exercise-induced haemorrhage for over 300 years. Almost all competing horses develop exercise-induced pulmonary haemorrhage (EIPH) at some point but most horses don?t suffer from reduced competing capacity due to the disease. The cause of the exercise-induced haemorrhage is unknown. The most believed theory today is that EIPH is induced by pulmonary stress failure caused by pressure over 100 mmHg in the pulmonary arteries during exercise.

5-hydroxytryptamin (5-HT) is biochemically derived from Tryptophan. Kynurenic acid and quinolinic acid are also known metabolites of tryptophan. 5-HT is considered to play a role in depression and today selective serotonin reuptake inhibitors (SSRIs) are used for treatment of depression. 5-HT may also play a role in pain perception. The aim of this study was to investigate whether a supplement of tryptophan may affect conditions such as depression and/or pain.

Pyometra, chronic purulent metritis in metoestrus, is a common disease in intact bitches. About 25% of all bitches in Sweden have contracted the disease before ten years of age. Clinical signs of the disease are varied, and the course may be either slow or acute. The diagnosis is most commonly made by radiographical or ultrasonographical examination of the abdomen in combination with blood biochemical and hematological analyses. The safest and most efficient treatment of pyometra is surgical ovariohysterectomy.There is room for improvement of both diagnostic tools and possible prognostic indicators for pyometra.Mast cells are large leucocytes, with granule that contain Inflammatory mediators.

Rheumatoid arthritis is an autoimmune disease, where joints are attacked by the own immune system, leading to chronic inflammation and destruction of bone and cartilage. Inflammation is a complex process, controlled by many different substances. One of them is adenosine, which has anti-Inflammatory properties. In this project, three polymorphisms in different genes, involved in synthesis and signaling of adenosine, were genotyped for 188 patients with RA and 362 controls without RA. The results shows that for the polymorphism in A2a, a gene coding for an adenosine receptor, there was no significant difference in genotype distribution between the groups.

Lower urinary tract diseases are a common problem in cats all over the world. The symptoms are for example dysuria, pollakuria, stranguria, periuria and hematuria. In approximately two thirds of these cats the underlying cause is not known and the disease is therefore called feline idiopathic cystitis (FIC). We need to know more about the disease to get a consistently effective treatment and prevention. A retrospective study was performed in 50 cats with symptoms of FIC and where other causes of inflammation were ruled out. Data like breed, age, sex, body weight, feeding, therapy and therapeutic results were collected, also if it was an indoor or outdoor cat, duration of the symptoms, if there was any stress and if the cat was living with another cat.

Bakgrund: Inflammatoriska tarmsjukdomar innefattar Crohns sjukdom och ulcerös kolit. De senaste åren har en ökning av de kroniska sjukdomarna skett bland annat i Sverige. Sjukdomarna går i skov och drabbar olika lager av tarmens slemhinna. För att hjälpa personerna att uppleva hälsa, trots kronisk sjukdom, bör sjuksköterskan känna till hur de upplever det att leva med sjukdomen. Syfte: Syftet med litteraturstudien var att beskriva personers upplevelse av att leva med en inflammatorisk tarmsjukdom.

Traumatic arthritis is a common cause of lameness in horses. One of different treatment alternatives of arthritis, is intra-articular injection with hyaluronan. As knowledge about pathophysiology of arthritis and function of hyaluronan is increasing, understanding grows about the mechanism of hyaluronan action in joints. Hyaluronan has, in addition to effects on viscosity, anti-Inflammatory and analgesic effects. Extensive research and scientific studies have been made on exogenous hyaluronan.

The main purpose of this study was to investigate the effects of the non steroidal anti-Inflammatory drug (NSAID) meloxicam in connection with dehorning of goat kids. Six 2-3,5 weeks old dehorned kids were given daily intramuscular injections of meloxicam (0,5 mg/kg; MG) and five age-matched dehorned kids were given isotonic NaCl (NG) for 3 days. The study was randomized and double blinded. The behavior of the kids was studied one day before dehorning, on the dehorning day and three days after. Blood samples were collected each day before the treatment and analyzed for plasma concentrations of meloxicam, hematocrit, cortisol, progesterone and glucose.

Bakgrund: En kronisk oförutsägbar sjukdom som Crohns sjukdom framkallar fysisk, psykologisk och social stress. Sjukdomen påverkar vardagen, försämrar livskvalitet och skapar oro. För att bedriva personcentrerad vård krävs insikt i hur denna oro upplevs.Syfte: Syftet med studien var att belysa sjukdomsrelaterad oro hos personer med Crohns sjukdom.Metod: Åtta kvinnor och fyra män mellan 30 och 64 år som skattat sin sjukdomsrelaterade oro som hög eller mycket hög identifierades. Intervjuer genomfördes och analyserades med kvalitativ innehållsanalys.Resultat: Analysen genererade tre kategorier: (1) Oro för sjukdomen i sig, (2) känslor runt oron, (3) samt hantering av oron. Sjukdomens oförutsägbarhet samt nedsatt funktion till följd av trötthet och bristande kontroll över tarmfunktionen var de mest framträdande orsakerna till oro.

Immune stimulating complex (iscom) matrix is a formulation that is used as anadjuvant within research. The capability of the iscom matrix to elicit an immuneresponse when not present together with an antigen has not been extensivelyinvestigated. One way to evaluate the status of the immune system is to measure theamount of cytokines that usually are produced by various cell types during an immuneresponse. The aim of the present study was to evaluate the early immune responses inporcine peripheral blood mononuclear cells (PBMC) after stimulation in vitro with theiscom matrix, AbISCO®-100. The immune response was evaluated by measuring themRNA expression for various cytokines, a chemokine receptor (CXCR4) andregulator of G-protein signaling (RGS16) in porcine PBMC after stimulation withAbISCO®-100 or other inducers like a lipopolysaccharide (LPS) and a CpGoligodeoxynucleotide (ODN 2216) used as controls.

In Sweden regulations stipulates that dairy cows are kept on pasture, or given the opportunity to spend time outside during a coherent period of between two to four months, depending on region. The transition out to pasture is a big change from the winter period in the stable, especially for the modern high yielding dairy cows. The let out to pasture includes a change in feed, environment, and new routines. There are indications that the milk somatic cell count (SCC) rises in a peak shortly after the let out. The purpose of this study was to see if peaks in the SCC could be observed after the let out, and to study the contribution of Inflammatory cells (neutrophils) and if the milk composition are affected. The study included 35 cows that were kept in stable during the winter.