Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

In recent years, moisture damages have been noticed in rendered, undrained stud walls. The design is built on the principle one-stage tightening which means that there is no air gap in the construction. The damages have occurred when water has permeated through the rendering in leaking connections and fittings for windows, doors, canopies, balconies, terraces and awnings. Behind the plaster carrier, which consists of either polystyrene or rigid mineral wool, plasterboard has often been used as a wind protection barrier. In many cases the wind protection barrier and the underlying wooden studs have been exposed to mould and in some cases even rot.

Background;Chronic obstructive pulmonary disease (COPD) is a lung disease characterized by airway obstruction. Common signs of COPD are the slow process and shortness of breath on exertion. The most important treatment is to quit smoking. Most nurses, regardless of where they work, will meet these patients, often when the patients are having respiratory disorders. Aim;the aim of this study was to illuminate how patients with COPD describe the daily life based on how he/she experience and cope with the disease.

The aim of this study is to bring forward four teachers of Arts-education, their statements and reasoning concerning the condition and meaning of the subject of Arts-education along with their statements in relation to theory and earlier research, give voice and insight in their intentions according to what teaching of Arts-education should contribute to.The basis for the study is brought out of the dilemma that there, in the subject of Artseducation is ?crowding of matter? in the nine-year compulsory school, difficulties in selecting the importance of various subjects and different opinions between teachers, due to curriculum being modified in accordance with the development in society. The earlier research that this study relates to shows how teacher?s opinion of different subjects varies. My questions concerning the subject are the following;How do four teachers of Arts-education reason concerning the condition and meaning of the subject of Arts-education?What are the teachers intentions according to what teaching of Arts-education should contribute to?What are the similarities and differences between the statements of the teachers in this study versus the teachers' statements according to previous research?To find answers on my issues and questions I have made interviews with four teachers of Arts-education and have then put their reasoning and statements against theories , which  all concerns the creative process.

In the current study, the aim was to investigate whether tail posture and motion can be an indicator of the emotional state of pigs and if the tail posture of the pig is affected by social breeding value (SBV), coping style and/or housing. Emotional state can be defined in two dimensions: valence and arousal. Two batches of 96 finishing pigs were studied in a one generation selection experiment with a 2x2 set up and were housed in a barren or straw bedded pen. In each pen, 6 pigs (3 male, 3 female) were housed. A back-test was done to determine the coping style of the pigs with two categories; high resister and low resister pigs.

Ten colic horses and seven healthy horses were included in a prospective study aiming to evaluate acid-base disturbances in colic horses. Traditional acid-base methods and measurements of lactate were used to study prognosis and changes before, during and after surgery. Strong ion gap, anion gap and base excess were compared to evaluate their ability to predict lactate. Of the ten colic horses that went through surgery, seven survived, two were euthanised and one died. Significant differences in plasma lactate levels were seen between the group of survivals and non-survivals, p=0,000025. No differences were seen in pH and base excess between the two groups.

The purpose of the present study was to examine effects of driver, vehicle, and environment characteristics on Collision Warning System (CWS) design. One hypothesis was made that the capability of collision avoidance would not be same among a driver, vehicle, and environment group with different characteristics. Accident analysis and quantitative analysis was used to examine this hypothesis in terms of ?risk? and ?safety margin? respectively. Rear-end collision had a stronger focus in the present study.

The purpose of this project was to investigate if retention of polar compounds that are given to treat tuberculosis, diabetes, inflammatory bowel disease and childhood leukemia could be obtained with HILIC separation. By varying different parameters for different types of columns the compounds were analyzed with the aim of finding guidelines for future method optimizations. To perform these analyzes three different columns were tested ? ZIC-HILIC (silica-based with zwitterions), ZIC-pHILIC (polymer-based with zwitterions) and XBridge Amide (amide functions). The results were evaluated with selected quality measures.

Background: Cystic fibrosis (CF) is the most common hereditary disease that leads to an early death. Earlier CF was considered as a childhood disease but today the expected age is 50 years. CF affects all the epithelial cells in the body which makes mucus and other body fluids more viscous than normal. The thick mucus leads to among other things respiratory problems and infections of the lungs. Today there is no cure, only medicines that controls the symptoms.Aim: To describe parents experiences living with a child with CF.

Leber´s hereditary optic neuropathy (LHON), a disease affecting vision, is caused by several point mutations in mitochondrial DNA. Mutations leading to a defect NADH ubiquinone oxidoreductase protein will affect the respiratory chain and cause a disturbed ATP production. It is still unknown why this defect leads to the degeneration of retinal ganglion cells and cells in the opticus nerve as well as demyelination of axons in these areas. Analysis of mitochondrial DNA is an important tool in the diagnosis of the disease. At the present time analysis is based on cleavage by restriction enzymes, which only detects two of the most frequent mutations: m.3460G>A and m.11778G>A.

In the year 1783 a fissure eruption from Laki in the southern part of Iceland produced a large amount of volcanic gases during a period of eight months. The volcanic gases and aerosols spread across the northern hem sphere due to ideal weather conditions and had a substantial impact on the environment, human health and also climate effects. This, so called dry fog, caused severe health problems, which led to death casualties, killing animals, crops and other vegetation. The consequence was a widespread famine which was aggravated by a hard and long winter in 1783/84. Analysis shows that the Icelandic volcanoes are the primary risk to produce dry fog that can affect Sweden.

Träningsinducerad lungblödning, Exercise-induced pulmonary hemorrhage (EIPH), är ett stort problem hos travare och galoppörer i hela världen. Det får anses klarlagt att mekanismen bakom EIPH är så kallad stress-failure, utmattningsskador, i blod-gasbarriären vilket beror på att hästarna har en respiration och cirkulation som ger extrema påfrestningar på denna sköra struktur. En bidragande faktor till stress-failure tycks kunna vara att hypertension i lungvenerna leder till att dessa remodelleras och förtjockas, vilket i sin tur leder till ännu högre tryck i lungkapillärerna. Den här litteraturstudien handlar om hur inflammation i lungorna kan ge upphov till EIPH. Inflammation finns med i problematiken runt EIPH både som en bakomliggande faktor och som ett resultat av blödningen.

Thoroughbred racehorses have suffered from exercise-induced haemorrhage for over 300 years. Almost all competing horses develop exercise-induced pulmonary haemorrhage (EIPH) at some point but most horses don?t suffer from reduced competing capacity due to the disease. The cause of the exercise-induced haemorrhage is unknown. The most believed theory today is that EIPH is induced by pulmonary stress failure caused by pressure over 100 mmHg in the pulmonary arteries during exercise.

The mainpart of this thesis is about the developing of a new analysis tool to be used in Zenicor Medical Systems AB ECG-system. The primary task of the system is to simplify the ECG survey for patients who suffers from different kinds of arrythmias, for example heart fibrillation. With this system is it possible for the patients to do their ECG survey by them self at home and then send the signal with their telephone or mobilphone to a server. The equipment used to do the survey is not bigger than you can have it in a pocket and this results in a bigger flexibility for the patient. A doctor can connect to the server and analys the ECG-curve and follow up the patients condition..

Swedish farmers have the possibility to be subsidized by EU rural development programme for grazing animals on pastures to promote the biodiversity of flora and fauna. The environmental remedy sometimes does not allow supplementary feed and at the same time the Swedish Animal Welfare Act and the Swedish Agricultural Board?s regulations for animal welfare and protection states that animals, in this case horses, shall be fed with food of good quality and adapted for the species. The regulations also state that they should have an individual feeding regime that enables them to maintain a normal body condition. The aim with this master thesis has been to investigate if it is possible to keep Gotland ponies on extensive pastures, during late spring and summer and still maintain animal welfare. The horses were assessed every fourth week using a welfare assessment protocol developed in line with the Welfare Quality® project (submitted, Viksten et al) where physical, behavioural and resourcebased parameters were observed. Twelve one-year old stallions of the breed Gotland ponies purchased from different breeders were used in the study.