The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

The purpose of this study is to map out and to analyze the spatial variation of female representation at the national level in established democracies. The aim is also to explain the variation in the female representation. The main questions that the paper tries to answer are:1.How does women representation in established democratic parliaments vary?2.Why does women representation in established democratic parliaments vary?This study is a comparative and statistic study, i.e. a study that includes a bigger number of countries and where quantitative analysis methods are used in order to achieve comparative analyses.

In Sweden plans to build high speed railways have been around since the early 1990s and the planshave developed to be more precised since. The future Swedish high-speed rail network is supposedto consist of two lines. Götalandsbanan; from Stockholm to Gothenburg, and Europabanan fromJönköping through the Öresund Region to Hamburg.The description of the aim to build high-speed railways in Sweden has in former reports always beento achieve rapid passenger traffic between big Scandinavian cities and Europe. There are almostnothing mentioned about the possibilities of high-speed transports of cargo on these railways. Thisstudy aims to clarify the freight transport market for Götalandsbanan and Europabanan.Today there is only one transport system with high-speed freight trains.

This thesis suggests an explanation for how it is possible for individuals living in an unsafe neighborhood to expressa highlevel of security. Araby is the district in Växjö municipality withthe highest level of insecurity. Within the district, there is acategory of individuals in security-measurements showing a high degree of security. Inhabitants living in house in Araby also seem to show a higher level of security than inhabitants in the same accommodation in other neighborhoods in the municipality.The study's purpose is to understand how individuals belonging to an unsafe neighborhood can experience a high degree of security.Qualitative data were collected from an observation in Araby and from interviews with individuals living in house in the district.Previous research has shown that security in the surrounding area depends on several factors, such as disorders, the degree of social control and personal opinions. Thestudy shows how a distinction to places and people in the district can explain the degree of security.

This document presents calculations of adjusted GDP based on satellite household accounts for the year 2001 with the purpose to compare adjusted and official GDP for Sweden, Germany, Estonia and Finland. Household production in each country is estimated using time use survey data from Eurostat and a monetary value is estimated using average wage (after taxes) for each country. The results indicate that the value of household production using this methodology increases GDP with between 42% (Finland) and 55% (Germany) compared to official GDP statistics. A comparison of GDP/capita between the countries included in the study shows that the GDP/capita in Germany increases more that the other countries due to the relatively high proportion of time used for household production (mainly German women) combined with a high average wage. In fact, the results show that Germany has higher adjusted GDP/capita than both Finland and Sweden (but Germany has lower GDP per capita when official statistics is used for the comparison).

The purpose of this paper is to try to get an understanding of the underlying causes and reasons that students starting in seventh grade junior high school seek out a particular group, and the extent to which they do so.The work is based on the questions: How do students perceive the meeting with their prospective bench-companion in a new composite class? What should a teacher consider when it comes to bench placement in a new composite class? Do the students change, and if so to what extent, friends when they start high school? What are the factors that affect/have affected the students' choice of friends? How large a role does the school play for pupils relationships? What thoughts and feelings do the students have regarding new encounters?I have conducted in-depth interviews with six adolescents in grade seven as the basis for the understanding of how students relate to group selection and group affiliation, and I have then compared this material with previous research on the subject. Furthermore I have availed myself of literature in social- and group psychology.The conclusion which has emerged is that the most important component of a group-process is the sense of solidarity and security, and the results show that students largely retain their old relationships rather than adding new, when they start high school. Hence the teacher plays an important role in ensuring that any imbalance in the group is offset and make sure that nobody is excluded, but also to help make room for new groups and be aware of the sensitive and responsive phase of life that the young people are in..

High technology companies in Sweden spend a huge amount of money on research and product development in proportion to what they get back in earnings from their inventions. Product development has traditionally been regarded as much more important than business development. This thesis aims to point out the critical factors behind successful business development processes for high technology companies. We have chosen to make a case study of Hexagon, a highly successful and innovative company within the measurement technology business. Internal and external actors that are important for business development have been identified and interviewed in order to understand how ideas are developed and executed.

AimThe purpose of the study was to investigate how upper secondary school students experience performance demands during their physical education and health classes (PE-classes). The questions were as following: Are there differing perceptions between the sexes whether students experience performance demands? In what situations do the students feel that the demands on their performance are high during PE-classes? Do the students experience of demands on performance differ whether the physical education and health teacher is male or female? Do students feel unfairly treated by the physical education and health teacher during PE-classes?MethodSeven upper secondary schools in Stockholm?s administrative district were randomly selected to participate in the survey. The principals were contacted for further information and to set a date for performing the survey. The project leaders were present during the days the surveys were taken by students in the social science and natural science programs.

Sweden's drinking water quality is considered to be high partly due to a high quality of the raw water and a well developed sewage infrastructure. Despite this, there is water contamination that could be prevented by installation of a sophisticated early warning system. Some of the major players in the production of drinking water have already invested in different types of early warning systems to ensure drinking water of high quality. There are various forms of early warning systems where automatic monitoring of E. Coli is an interesting alternative.

The purpose of this thesis has been to gather knowledge about the complaints of tying-up in horses and to show that the importance of feeding. The thesis includes a literature review of tying-up how preventative measures by feeding can be made. In the equine sport, crosscountry, horses occasionally suffers from tying-up, a muscle disease. An increased knowledge about the disease is demanded by riders and trainers as the disease disrupts training and competition of suffered horses. A cross-country horse works very hard in training and during competitions but the work bouts are long enough for the muscle cells to use aerobic pathways in the metabolism.

The gaming market has become the fastest growing market for leisure and is one of the most popular forms of homeentertainment. This has generated a media debate about how people are affected by gaming and concernes how we are affected by the violence that may occur in games. Games proponents argues that gaming is the future of teaching and computer games are becoming a sustainable and popular instruction strategy. Some studies have long used computer games for educational purposes. By interviewing high school students in focus groups as qualitative research, I studied their attitudes towards computer games as a learning tool.

Purpose ? The current study aims to analyze how employees in temporary groups gets motivated, based on their personality traits and generation belonging, focusing on the goal setting theory. The study also focuses on the combination of these two variables concerning the goal setting theory.Design ? A total of 56 individuals working in project groups completed the questionnaire made for measure their personality traits and their work motivation.Findings ? Results in this specific case demonstrated differences in motivation based on their personality traits and generation. The study also found differences when studying the combination of the two variables.

The overall purpose of this essay is to come to a deeper understanding about videos in the Youtube beauty community by using a mixture of qualitative and quantitative methods. By answering the main questions of the essay which are; What quantitatively measurable components are there in the videos that can be seen as contributing factors leading to the fact that some generate more views that others?; Is there a set mold for success containing certain aspects that a video must have to stand out?; What correlations can be found between the two categories of analysis and how do these potential correlations affect the popularity of the video? and What effects do specific components on the level of popularity of the video?, we?ve been able to distinguish differences as well as similarities on both qualitative and quantitative levels. We have also seen that using the two methods together have enabled us to reach more profound results. The main result that we have found on a quantitative level is that there are obvious correlations between a high level of popularity and a good visual quality.

The aim of this study was to map out the quality of the treatment in the forensic outpatient care unity in Malmoe from a patient point of view. This target group, seriously mentally disordered law-offenders, has been neglected in the scientific research. This investigation will hopefully contribute to a positive change in this subject. To achieve a good understanding of this field a methodological triangulation was conducted which included an observational study, a pilotstudy and semistructured interviews. 14 patients and 10 staff members were interviewed.

Although school librarians have an important role to play in high schools helping teachers and students during their education, Swedish school librarians are often considered to end up left out by teachers and head principals due to school librarians vague and unclear professional role in the school. The aim of this thesis is to attempt to study school librarians own apprehensions of their particular professional role in a high school context, and how their professional role is believed to be apprehended by teachers and head principals. The results are based on interviews made with eight school librarians working in different high school libraries in Gothenburg during April 2002. By using phenomenographic method various existing apprehensions of the specific phenomena that the professional role of the school librarian constitutes of are made clear. The aim of the method is not to study what the profession constitutes of, but rather how it is apprehended by the school librarians themselves.