Flowcytometric technology has been widely used for measurement of the absolute numbers of T-lymphocytes subsets in Human Immunodeficiency virus (HIV), defining the disease state, monitoring antiviral treatment, and identifying any risk for opportunistic infections. A manual preparing of the samples is required. More recently an automated and enclosed blood cell counting, Celldyn Sapphire has been introduced. In this study the performance of the Flow cytometer EPICS XL as a reference method for analysis of CD3+, CD4+ and CD8 T-lymphocytes was evaluated with blood from 40 individual?s samples.

FLUTD - Feline Lower Urinary Tract Disease ? is a complex disease and a common sight in the veterinary setting. Although FLUTD excluding urethral obstruction may be discomforting for the cat and its owner due to symptoms such as periuria, stranguria, hematuria, dysuria and pollakiuria, obstruction presents an acute and potentially lethal form of the disease as a result of uremia and disturbances in fluids, electrolytes and acid-base balances. A study of literature has been performed to investigate the causes of FLUTD with urethral obstruction and the actions available to the owner to avert them. Potential causes of obstructive and non-obstructive FLUTD have been found to include infections, uroliths, urethral plugs, neoplasia, anatomic abnormalities, trauma, neurogenic or iatrogenic causes. Most commonly a cause cannot be identified and the terms idiopathic FLUTD or feline idiopathic cystitis are used. As the mortality rate due to urethral obstruction likely is somewhere around 5.8-8.9% and FLUTD including and excluding urethral obstruction constitute great inconveniences, precautionary measures are warranted.

Background: Breast cancer is one of the most common cancer diseases among women worldwide. Breast cancer brings fear into many womens? life. It is a threat against life, but also to the psychological health, self-esteem and the female identity. Aim: The aim of this literature review was to describe newly breast cancer patient?s perception of information provided by healthcare personnel.

In this thesis the core of the nursing professional skill will be portrayed through the opportunities of the essay writing. Thus the tacit knowledge, which is at the verge of erosion in a zeitgeist where medical care shall be cost-efficient and where development is mainly based on scientific research, will be articulated. Scientific articulation of knowledge has gained increasing importance on the expense of the practical knowledge. Healthcare professional skill requires a refind ability of judgment, which absorbs its nourishment from both scientific knowledge and the proven proficiency expertise.Professional skill expresses itselfs through action, especially through the acting in unexpected incident. Experience is developed into knowledge through reflection.

It is self evident that our society is an information one. This is true from the things we see around us.The world is now a global village.It just take seconds for communication to be established from one part of the globe to another.What a tremendous achievement for information technology.Among the recent developments of information technology is the scientific wizardary of cyber medicine.The internet has definitely revolutionalised the healthcare industry. Many people in developed countries of the world seek medical information, advice or even buy drugs via the internet.So many websites rise every now and then claiming to provide various medical assistance to patients. But the application of information technology to medicine poses some ethical problems today. It is because of this that cyber medicine attracts my attention in this research.With this research, I hope to offer some recommendations for a morally acceptable cyber medicine .This will help to some extent in solving this all important problem ofcyber medicine for the good of the health care industry and the society at large..

Background: Patients with leg ulcer suffers often of woundpain. Healthcare professionals will strive after having knowledge and understanding for how woundpain influences the patient's quality of life. Aim: The aim is to elucidate the patient's experiences of pain at venous leg ulcer and the effects on their daily life. Method: The procedure has been a systematic literature overview with a systematic search in different databases. The search resulted in nine scientific articles.

This is a thesis performed at GEMS PET Systems AB in Uppsala. GEMS PET Systems AB is a company within GE Healthcare. GEMS PET Systems develops and manufactures equipment for manufacturing of radioactive isotopes for PET which is a scanner used in medical applications. The systems includes an accelerator of cyclotron type and other support equipment. One system is shipped in two or three containers depending on order configuration.

Diabetes type 2 is a fast growing illness and a global health problem, often a result from less appropriate standard of living. Lifestyle changes related to diet and physical activity are important lifestyle changes regarding to get control of the self-management and to avoid vascular complications.AimThe aim of this study was to describe patient's experiences of lifestyle changes in diabetes type 2.MethodA literature study was carried through based on 12 qualitative scientific articles.ResultsThe analysis result into two main themes and eight subthemes. Main themes where barriers and motivators for lifestyle changes and the subthemes where struggle, denial, lack of knowledge, alienation, support, motivation, acceptance and knowledge.ConclusionThis study shows that lifestyle changes are experienced different by patient with diabetes and nurses should therefore be aware of the unique and specific demands these patients require. This is important for providing a good diabetic healthcare and to help patients with their self-management.

The purpose of this essay was to see how the construction of elderly immigrants as a problem in healthcare and elderly care has been described in the relevant literature. In the course of doing so I tried to answer the following questions; What is said to be the expected problems or difficulties in work with eldely immigrants? How are the expected members of the problemgroup defined and identified? Whos perspective dominates the description of the problem, the users or the investigators? These questions were hard to answer and I had to conclude that the problem was not clearly defined; there was only an assumption of a problem. This, however, has not been a hindrance in the category of elderly immigrants being claimed to be a group with problems that need research and further investigation. The dominating perspective is that of the investigators as they are the ones who define and identify who is a part of the problemgroup..

En förutsättning för att få folk att delta i en friskvårdssatsning är att de är motiverade. En kvalitativ studie genomfördes med syfte att beskriva och förstå den yttre och inre motivationen hos kommunanställda som deltar i en friskvårdssatsning på vattengymnastik. Femton respondenter intervjuades utifrån en intervjuguide baserad på Deci och Ryans (1985) fyra faktorer (självbestämmande, kompetens, samhörighet och glädje) som ses som en förutsättning för att motivation skall uppstå. Resultatet visade att begreppen vanemöster, kompetens, samhörighet/social kontakt samt glädje/välbefinnande var centrala för att uppnå motivation att delta i vattengymnastik. Studien antyder att en arbetsplats kan skapa yttre förutsättningar för att de anställda ska ha möjligheten att hitta en passande motionsform.

En förutsättning för att få folk att delta i en friskvårdssatsning är att de är motiverade. En kvalitativ studie genomfördes med syfte att beskriva och förstå den yttre och inre motivationen hos kommunanställda som deltar i en friskvårdssatsning på vattengymnastik. Femton respondenter intervjuades utifrån en intervjuguide baserad på Deci och Ryans (1985) fyra faktorer (självbestämmande, kompetens, samhörighet och glädje) som ses som en förutsättning för att motivation skall uppstå. Resultatet visade att begreppen vanemöster, kompetens, samhörighet/social kontakt samt glädje/välbefinnande var centrala för att uppnå motivation att delta i vattengymnastik. Studien antyder att en arbetsplats kan skapa yttre förutsättningar för att de anställda ska ha möjligheten att hitta en passande motionsform.

Bakgrund: Postoperativa sårinfektioner [PSI] är en allvarlig komplikation och ett hälsoproblem som orsakar lidande för patienten. Såsom vid alla operativa ingrepp förekommer en risk att få PSI i operationssåret/-såren efter Coronary Artery Bypass Graft [CABG], men det finns redan en rad kända riskfaktorer som ökar risken för PSI.Syfte och metod: Syftet med rapporten var att undersöka om kombinationen av ett antal sedan tidigare kända riskfaktorer ökade risken för PSI efter CABG på ett mellansvenskt sjukhus åren 2009-2012. En retrospektiv journalgranskningsstudie med totalt 228 patienter genomfördes.Resultat: Av 228 undersökta hade totalt 50 patienter rapporterat sårinfektion och 73 patienter hade ? 3 riskfaktorer. Bland de som hade ? 3 riskfaktorer rapporterade 32,9% PSI och bland de som hade < 3 riskfaktorer rapporterade 16,8% PSI.

Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

Background: Diabetes is a common disease where the degree of self-management is of paramount importance. Individual factors influences both cooping and general wellbeing among diabetic patients. Glycemic control is known to be important but other factors ought to be considered. Aim: The aim of this study was to describe how individual self-management influences the general wellbeing among adult diabetic patients. Method: This literature study is based on scientific articles.

Syftet för denna studie var att studera förekomsten av postoperativa sårinfektioner hos patienter som genomgått kärloperation med inguinal incision under åren 2008 till och med mars månad 2013. Undersöka skillnader gällande riskfaktorer inom studerad patientgrupp för förekomst av postoperativ sårinfektion samt studera om patienter som utfört endovaskulär metod under samma vårdtillfälle oftare drabbades av postoperativ sårinfektion än de patienter som inte genomgått endovaskulär metod under samma vårdtillfälle. Metoden som användes var granskning av patientjournaler efter utifrån studien utvalda specifika operationskoder. Granskningen av de 54 patientjournalerna skedde med hjälp av egenmodifierad tidigare använd granskningsmall. Studieresultatet visar att förekomsten av postoperativa sårinfektioner hos den studerade patientgruppen uppgår till 22,2 %.