Sweden signed, in 1992, the Convention on Biological Diversity (CBD) and has thus assumed responsibility for the indigenous biodiversity. That also means that Sweden has a conservation responsibility for the Swedish Friesian (SLB) (SJV, 2007a). In a survey conducted by the Board of Agriculture (SJV) in 1996, it appeared that there were around 780 cows and heifers of the SLB breed (SJV, 1997). SJV did in 2006 a plan for the development of livestock genet-ic resources and the number of cows of SLB was estimated at approximately 200. This meant that the SLB was in need of being conserved and SJV assumed it would be categorized as threatened.The primary objective of conservation of a breed is to reduce the loss of the original genetic variation (Lacy et al., 1995).

This review article presents the splicing process during messenger RNA maturation and how it is regulated by different Cis-regulatory RNA-sequence elements and splicing factors. A more detailed description of the process alternative splicing and its importance to the function of Genes from the model organism Arabidopsis thaliana is also given. A single eukaryotic gene can by the process alternative splicing (AS) give rise to a number of functionally mature mRNA-molecules, which in turn encodes for structurally and/or functionally different proteins. During the course of evolution, the process alternative splicing has thus shown to be effective in increasing transcriptome and proteome diversity of most eukaryotic organisms. This suggests therefore that the dominant theory in molecular biology, a gene encodes for a protein, needs to be corrected.

In most countries, the dairy industry focuses on the volume of milk produced instead of its composition. This results in a high content of water and a lower proportion of fat and protein. A high proportion of water is not desirable when processing the milk into various dairy products like cheese, cream and milk powder. High water content also increases the costs for transport and storage, and also an unnecessary, albeit marginal, strains on the world?s water reservoir.

Nematode-transmitted plant viruses (such as nepoviruses) cause great economically losses in commercially important plants such as tomatoes, grapevines and raspberries all over the world. To avoid spread of the viruses the interest in mapping the viruses has increased. One of the important nepoviruses infecting European red raspberries (Rubus idaeus) is Tomato black ring virus (TBRV). TBRV has earlier been confirmed in raspberry samples from Belarus by ELISA and the aim of this bachelor degree project was to confirm these results by amplifying, cloning and sequencing the coat protein (CP) gene of the virus. In total eight viral cDNA samples, from two different raspberry samples, were amplified and sequenced.

Perchlorate and chlorate are naturally occurring in the atmosphere, from here it sediments into groundwater and soil. The pollution is increased by discharges of perchlorate and chlorate from agriculture and paper mills. Bacteria capable of reducing perchlorate and chlorate to chloride and oxygen can be used to get rid of these contaminants. However an anaerobic environment needs to be sustained in order for this reaction to be used. For this reduction to work in an aerobic environment as well, a greater knowledge of the reducing enzymes, regulating factors and their corresponding Genes is needed.

In schools, misconceptions about genetics can arise due to several factors, and the consequences of these misunderstandings can be significant. Lack of education can play a central role in the spread of misinformation about genetics. When teachers lack sufficient knowledge or do not have access to up-to-date resources, they risk inadvertently conveying inaccuracies to students. Additionally, students own preconceived notions and misinterpretations can be reinforced through interaction with their peers, creating a vicious cycle of misinformation. In this study, various misconceptions within genetics education have been identified and analyzed, while different strategies to prevent these misconceptions have been examined. The study revealed that the two most common misconceptions are the confusion between "Genes" and "DNA" and the simplified perception of genetic determinism.

It becomes more important to consider different behaviour traits in the selection for dairy cows to be suitable in the production systems of today. The purpose of this investigation was to study the genetic background to behavioural traits and the heritabilities for temperament, rank order, aggressiveness, feeding behaviour and for learning behaviour. Moreover, the genetic correlations to other traits were studied. To be included in a breeding programme a trait needs to have enough genetic variation, a moderate to high heritability or to be correlated to another measurable trait. Behaviour is a quantitative trait affected by many Genes and the environment.

The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 Genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

Leber´s hereditary optic neuropathy (LHON), a disease affecting vision, is caused by several point mutations in mitochondrial DNA. Mutations leading to a defect NADH ubiquinone oxidoreductase protein will affect the respiratory chain and cause a disturbed ATP production. It is still unknown why this defect leads to the degeneration of retinal ganglion cells and cells in the opticus nerve as well as demyelination of axons in these areas. Analysis of mitochondrial DNA is an important tool in the diagnosis of the disease. At the present time analysis is based on cleavage by restriction enzymes, which only detects two of the most frequent mutations: m.3460G>A and m.11778G>A.

Syftet med denna litteraturstudie är att genom granskning av vetenskaplig litteratur undersöka hur de riskfaktorer i arbetet som orsakar utbrändhet bland sjuksköterskor kan förebyggas. Frekvensen av utbrändhet i Sverige har ökat sedan slutet av 90-talet och de riskfaktorer som har identifierats av olika forskare är av organisatorisk, individuell och social Genes. För att uppnå syftet gjordes en litteraturstudie som grundar sig på åtta vetenskapliga artiklar. Som teoretisk referensram valdes Demerouti´s Job Demand-Resourcesmodell för att tydliggöra begreppet utbrändhet. Mätinstrumentet MBI användes också för att förklara hur utbrändhet kan mätas.

Anthelmintic resistance is a major problem in sheep husbandry all over the world. One way toslow down the development of anthelmintic resistance is to keep part of the parasitepopulation in refugia (unexposed to drugs) which will maintain the Genes for susceptibilitywithin the population. Climate, type of parasite and drenching regimes effect the size of therefugia. Dilution of resistant with susceptible parasites, targeted treatment and targetedselective treatment are all management strategies that employ refugia. Dilution is possible butcomplicated.

The objectives of this study was to examine the prevalence of hypertrophic cardiomyopathy in a family of British Shorthaircats to determine the mode of inheritance and to investigate if the disease was associated with a mutation in myosin binding proteinC (MyBPC3). The family comprised 28 cats, seven male and 21 females. The cats underwent a physical examination, including cardiac auscultation, and an ultrasound examination. Blood was collected for DNA- analysis. The blodsamples were sent in a buffer solution to Kathryn Meurs, Washington State University, USA for analysis, where the Genes coding for MyBPC, Troponin I and T were characterized using microsequencing technique. Out of the 28 cats, 8 were diagnosed with HCM, 2 were diagnosed with congenital heart disease but had no evidence of HCM.

Genetic defects are caused by mutations in major Genes where the gene?s protein product has a large impact on the physiology of the animal. The synthesis of the protein can be altered by a change in the nucleotide sequence, which can lead to malformation and in many cases death.One of the main reasons of increase in many genetic defects is the use of few bulls in breeding programmes, causing a reduction of the genetic variation. Genetic defects cause suffering for the animal and influences the production by, for example, increased costs due to misscarriages, lost milk production and expenditure for medical treatment. Bovine Leukocyte Adhesion Deficiency (BLAD) and Complex Vertebral Malformation (CVM) are two genetic defects that were widespread before the causative mutation was discovered.

Rhodesian ridgeback is a dog breed that originates from southern Africa. The characteristic ridge (a dorsal ridge where the hair grows in the opposite direction to the general coat) is shared with an Asian breed, Thai ridgeback. The origin and inheritance of the ridge has been examined and defined. The ridge-mutation is a duplication that contains four complete Genes, FGF3, FGF4, FGF19 and ORAOV1 and the 3´-end of CCND1. The ridge is inherited as an autosomal, dominant trait and predisposes for Dermoid sinus (DS), a disease that develops during embryoGenesis.

Rheumatoid arthritis is an autoimmune disease, where joints are attacked by the own immune system, leading to chronic inflammation and destruction of bone and cartilage. Inflammation is a complex process, controlled by many different substances. One of them is adenosine, which has anti-inflammatory properties. In this project, three polymorphisms in different Genes, involved in synthesis and signaling of adenosine, were genotyped for 188 patients with RA and 362 controls without RA. The results shows that for the polymorphism in A2a, a gene coding for an adenosine receptor, there was no significant difference in genotype distribution between the groups.