Alternative splicing is a process that partly rejects the common definition of a Gene ? that one Gene codes for one specific protein. By variable combination of coding regions (exons) and exclusion of non-coding regions (introns), formation of several different mRNA-transcripts, and consequently several different proteins, can derive from the same Gene. Alternative splicing is an important condition for the development of complex life forms, but it is also a highly sensitive process and inaccurate splicing is the cause of approximately 15 % of mutations that cause Genetic diseases. This article presents four Genes, BRCA1, BRCA2, ER? and ER?, and inaccurate splicing of these Genes increases the risk of developing cancer, particularly breast cancer and ovarian cancer.

Canine dilated cardiomyopathy (DCM) is a disease with high morbidity and mortality and with a high prevalence in certain breeds. Predominatly large breeds are affected, such as Great danes, Newfoundlands, Deerhounds and Dobermanns. One exception is Cocker spaniels, where both American and English Cocker spaniels are affected in a quite high frequency. Cardiomyopathy is characterised as a disease that affects the myocardium and gives an impaired heart function. To diagnose DCM following criteria have to be observed: 1. Dilation of the left ventricle. 2. Reduced systolic heart function. 3. Increased sphericity of the left ventricle. To be certain of the diagnosis, alternative reasons for the symptoms such as lung- and heart diseases with other etiologies have to be excluded Two different types of DCM have been found upon histological examination.

The escalating usage of pyrethroids has resulted in an increased awareness about resistance towards pyrethroids in insects. Pyrethroids inhibit voltage-sensitive sodium channels (VSSC) in nerve cell membranes and are composed of synthetic molecules based on pyrethrins present in pyrethrum extracts from Chrysanthemum species. VSSC are transmembrane proteins that are important for electric signalling over the membrane in insects. Mutations in the Gene encoding the sodium channel have proved to be a common reason for resistance against pyrethroids. Pyrethroid resistance among pollen beetles is spread all over Sweden and also abroad and is increasing.

Promoters are Genetic elements that facilitate the transcription of a Gene and they have been found in front of non-coding RNA (ncRNA) Genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are Genetic elements that facilitate the transcription of a Gene and they have been found in front of non-coding RNA (ncRNA) Genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are Genetic elements that facilitate the transcription of a Gene and they have been found in front of non-coding RNA (ncRNA) Genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Gene technology has become more and more attractive in plant breeding during the last twenty years. This is probably because it can readily improve the existing commercial cultivars that have only one or a few drawbacks by modifying one or a few Genes without changing the main Genetic constitution. In this regard, Gene technology is more effective than conventional breeding.The aim of this project was to analyze the fruit quality of the non-transgenic apple scion ?Jonagold? grafted on non-transgenic and rolB-transgenic apple rootstocks. The rootstocks used were M9-rolB1, M9-rolB2, M26-rolB and non-transgenic controls M9 and M26.

A nonsense mutation in the DMRT3 Gene has been shown to have a large impact on pattern of locomotion in horses. Horses that can perform several other gaits in addition to the normally occurring gaits, walk, trot and canter, are often hetero (CA)- or homozygous (AA) for this nonsense mutation. Horses that only can perform walk, trot and canter are often homozygous for the wild-type Gene (CC). For example the Icelandic Horse is a gaited breed. Five-gaited Icelandic horses can perform both flying pace and tölt, except for the normally occurring gaits, walk, trot and canter, and are often homozygous for this nonsense mutation (AA).

Rotavirus and coronavirus are the two most common viral causes of neonatal calf diarrhea and their presence causes a lot of economic damage to the farmers as well as suffering to the animal. By getting better knowledge about the viruses we may help in tracing transmission and in producing new vaccines. This paper served to study the molecular epidemiology of rotavirus and coronavirus in fecal samples collected from two different farms in the state of São Paulo, Brazil. These samples have been studied with focus on the Gene VP4, VP7, NSP4 in rotavirus and the Gene S1 in coronavirus. From the first farm the screening PCR showed that 12/48 samples were positive for rotavirus and 4/48 positive for coronavirus.

Totally 84 differentially expressed mRNA clones from infective L3 larvae of the parasite Haemonchus contortus, a blood sucking nematode, were analyzed with single strand hybridization assay (SSH). Altogether 79 clones were sequenced, edited, and compared with proteins found via BLAST in GeneBank. The aim was to investigate Gene expression and potential protein expression following storage at 5 °C for 32 weeks. mRNA was extracted from fresh and stored L3. The SSH derived products were cloned into E.

The Swedish Red Polled breed is threatened by extinction and there are only around 1100 animals left. The breed was popular in the early twentieth century, but since then the numbers have steadily decreased. The overall objective of this study was to find out whether there may be economic incentives for preserving the breed. The milk quality properties are of major importance in the production of dairy products. Therefore we would like to establish if the Swedish Red Polled breed carries favourable alleles at loci of relevance for milk quality, to potentially make the breed more interesting for milk production.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific Gene at a given point in time and thereby also information of how the Gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

In 1993, Sweden signed the Convention on Biological Diversity and thus agreed to conserve our indigenous biological diversity. As a part of the convention, Sweden is obliged to conserve a number of domestic animal breeds and among them the Linderöd pig. In 1952, the zoo, Skånes Djurpark, took care of a pregnant sow that was regarded to be related to the old "forest pig". The old forest pig was thought to have been extinguished by the early 20th century as a result of the ban on boars not approved by state inspectors. However, according to later accounts, several farmers still kept some of these non-approved pigs.

African swine fever, ASF, was first described 1921, in Kenya. The virus has thereafter spread through many African countries as well as in Europe, Asia, South- and Central America. The virus is spread via three different cycles, of which one is sylvatic and includes warthogs and soft ticks. The other two are between domestic pigs and the one most common one is caused by humans, who not always realize the seriousness of precautions. The infection manifests as hemorrhagic fever, but can also be more or less unnoticed. The aim of this study was to use molecular tools to detect and partly Genetically characterize ASFV both in soft ticks and in serum from domestic pigs in Uganda. Comparing viruses detected in ticks with those detected in domestic pigs I also wanted to investigate possible links between the sylvatic and domestic cycle.

Titel: Genusframställningen i Star Trek ? en utopi eller dystopiFörfattare: Gustav Ekholm och Torbjörn KarlssonHandledare: Malin NilssonExaminator: Veronica StoehrelTyp av arbete: C-uppsats i Medie och Kommunikationsvetenskap, 10p HT-06Plats: Högskolan i HalmstadSyfte: Uppsatsen syfte är att fastställa huruvida åtta olika avsnitt ur tv-serien Star Trek belyser genusfrågor och utmanar avsnitten i så fall de rådande genusdiskurserna.Metod: Den här uppsatsen fokuserar på åtta Star Trek avsnitt som producerades mellan 1960-talet och 2000-talet. Metoden för analysen är en kritisk diskursanalys med utgång från Norman Fairclougs diskurskritiska modell.Resultat: Uppsatsens slutsats är att Star Trek-serierna berör genusfrågor i olika utsträckning beroende på vilket årtal avsnitten är från. Det konstateras också att viktiga genusämnen, likt homosexualitet, tas upp men beroende på olika omständigheter saknas ofta ett djup i framställningen.Nyckelord: Star Trek, Gene Roddenberry, science fiction, genus, diskurs, diskursanalys, textanalys, makt, tv-serier, dominansförhållanden..