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213 Uppsatser om Fluorescence in situ hybridization (FISH) - Sida 4 av 15
Full Circle: upstream and downstream migration of Atlantic salmon (Salmo salar) in the northern Swedish river Vindelälven
The life cycle of the anadromous Atlantic salmon (Salmo salar) can span large geographic, political, and socio-economic boundaries. Management strategies and regulations that only concentrate on small spatial scales often overlook larger basin-wide problems, especially relating to post-spawn seaward migration. In this master thesis one entire migration cycle of wild adult Atlantic salmon in the unregulated northern Swedish river Vindelälven was monitored by radio-telemetry tracking during upstream spawning migration and downstream seaward migration back to the Baltic Sea. The effect of ladder passage variables (time to pass, total time in the ladder, passage day over the ladder) at a fish ladder downstream in the river Umeälven, as well as fish size, were evaluated to determine if differences in upstream migration distance in the river Vindelälven could be observed. Ladder passage variables did not affect migration distance, but size exhibited a negative relationship to migration distance.
Threats and opportunities for the survival of the Yangtze finless porpoise
The Yangtze finless porpoise (Neophocaena phocaenoides asiaorientalis), is a small freshwater cetacean which has recently fallen dramatically in number. It is an endemic species for the Yangtze River and unless drastic action is taken, it risks the same fate as the now functionally extinct Yangtze river dolphin (Lipotes vexillifer). Threats to the porpoise are diverse but they have in common that they are caused by man. Development of the river has been made to supply a growing human population with food, hydropower, and to reduce the risk of flooding. These changes have resulted in a degraded habitat for the porpoise.
Vattenflödets betydelse för fångster av lekvandrande lax (Salmo salar) och öring (Salmo trutta) : En utvärdering av laxfällan vid vattenkraftverket i Forshaga
Many watersheds have lost stocks of migrating fish species because of the development of hydroelectric plants and other fragmenting barriers. The remaining stocks of migrating Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) in River Klarälven, Sweden are five times smaller compared to one hundred years ago. A fish-trap at the hydroelectric plant in Forshaga has been vital for the continued existence of the stocks. This is the first hydroelectric plant of eight, blocking the way to the spawning grounds. Spawners are collected in the trap for both hatchery and transport by truck upstream and past seven more hydroelectric plants to the spawning grounds, where they are released for possible natural reproduction.
Tillväxtmönster och åldersstrukturer hos opåverkade bestånd av strömlevande Arktisk harr i Sibirien :
It is hard to find streaming waters where humans haven?t affected the fish populations or the
running water it self. In many streams the fish populations are over harvested and this results
in a lack of older and larger fish. This is also the situation for many populations of Grayling
(Thymallus spp.). To be able to develop management plans and to perform actions that
improve the situation for the grayling, it is therefore crucial to know what natural, by humans
unaffected populations look like.
Undersökning av stereo och dynamiskt perspektiv i horisontell fish tank virtual reality
Ett par av de trick som används i virtual reality för att få 3D-världen att verka mer levande är stereoskopi och ett dynamiskt perspektiv med head tracking. Tidigare undersökningar har visat att head tracking har större effekt på 3D-upplevelsen än stereo [1], i alla fall när bilderna visas på en vanlig datorskärm. Den här undersökningen har försökt att ta reda på om samma sak gäller då den virtuella världen projiceras på en horisontell bordsskiva. Intressant nog går resultaten från det genomförda experimentet tvärt emot all teori. Tyvärr kan inga konkreta slutsatser dras eftersom alltför många felkällor har uppmärksammats, men studien öppnar ändå upp för framtida forskning..
Samband mellan en längre tillväxtsäsong och en ökad smoltstorlek i Vänerområdet
AbstractThe purpose of this study was to see if there is a connection between smolt length from Brattfors salmon cultivation and temperature. Climate changes are expected to give a higher temperature that could result in a longer growing season for the fish.Cultivated smolt is today bigger than it was before, it is also bigger than the wild salmon. The growth season of salmonids depend on the water temperature, salmonids need 6-80C to grow. Water temperature has been surveyd since the late 50´s in Brattfors. The result shows that it?s now considerable more days per year with higher temperature.
Naturvärdesbedömning av Utanåfladen; En inventering av vårlekande fisk, bottenfauna och vegetation
There are several thousands shallow bays along the Swedish east coast. Most of them are disturbed by different human activities, and undisturbed bays can be considered as quite unique. In the sheltered shallow bays water warms up more rapidly than adjacent water favoring the growth of vegetation, which in turns is beneficial to spring spawning fish species like perch and pike. It is nowadays more common that coastal habitats are inventoried to facilitate future preservation and planning. The aim of this study was to investigate the shallow bay Utanåfladen (59° 53`31.38 N 19°00`44.90 E) in the north Stockholm archipelago and evaluate its habitat value.
Follikulärt microbiom hos friska individer : Detektion av bakterier och svamp med in situ hybridisering och immunofluorescens
Bakgrund: Det är viktigt att främja barns fysiska aktivitet, eftersom det leder till många positiva hälsoeffekter. Tyvärr är många små barn idag inaktiva och når aldrig den rekommenderade gränsen på 60 minuters fysisk aktivitet per dag. Syfte: Att beskriva hur fysisk aktivitet kan främjas bland barn, i åldrarna 2-8 år. Metod: Studien som utfördes var en litteraturstudie där tio vetenskapliga artiklar granskades. Resultaten sammanfattades genom att söka meningsbärande enheter och därefter skapades teman av dessa.
Predation på evertebrater under tidig vår i sjön Tåkern
Benthic invertebrates play important roles as feeding resources for many organisms in different food webs. Shifts in predation of these organisms can generate cascading effects and potentially lead to the disappearance of one or more species from a site. Cascading effects can bring impacts to organisms who aren?t even directly involved, why studies in this field are important for understanding sudden changes in ecosystems. I examined the predation from fish and waterfowl on benthic invertebrates in the shallow and eutrophic Lake Tåkern in the plains of Östergötland County, southern Sweden.
Beteende inför lek hos odlad respektive vild lax (Salmo salar L.) i Klarälven
In many regulated rivers, authorities have stocked hatchery salmon to compensate for loss of wild populations. However, hatchery fish have not always behaved as wild fish do, and stocking them has not always fully compensated for the loss of wild fish. The purpose of this study was to compare swimming behaviour in the river before spawning, the choice of spawning areas and body size between wild and hatchery-reared salmon in the river Klarälven. Salmon behaviour in the river before spawning was analyzed using radio telemetry. The salmon were caught at Forshaga power plant, radio-tagged and returned to Klarälven at Ekshärad, 107 km from the river?s mouth.
Utvärdering av den gröna smileyn inom livsmedelsinspektion : En intervju och enkätstudie i Halmstads kommun
With a growing population on earth the amount of end-of-life vehicles (ELVs) and other scrap steel increases. The European Directive 2000/53/CE? requires a minimum of 95 % reuse and recovery and 85 % re-use and recycling no later than 1 January 2015, for end-of life vehicles. This imposes a need for more efficient recycling and recovery. Despite this shredder residue (SR) is commonly land filled.This report investigated the magnetic fines residue generated at Stena Metall shredder facility in Halmstad, Sweden.
Vattendragsrestaurering i teorin och fiskinventering i praktiken : en litteratur respektive metodstudie
Running water-pathways have played an important roll in Sweden and frequently been
manipulated. One profound change was made during the period of logging when
streams were cleared from obstacles as big boulders, sharp bends and side-streams.
Dams and different constructions gave control of the stream water and the streams
became channel-like with a homogen structure. This interference gave problems for
many fish populations and resulted in a shortage of feeding habitat, over-wintering
habitat, spawning habitat, unnatural flow regimes, sedimentation, unnatural
temperature fluctuations. Many of these historical changes of the watersheds are now
subjects for restoration, ie., bring back the streams to what we believed it was before the change. However, what once existed in terms of complexity is now gone and difficult toreplace.
Användbarheten av Tekla i Betongkonstruktioner : En jämförelsestudie mellan två modelleringsprogram
This thesis has examined the Tekla Structures modeling software and answered the question of whether it could act as a suitable modeling program for the design department at Bjerking AB in Uppsala. The method used in this work is a case study of a previous project with existing basis to work form. The demarcation of the study was to examine only the usability of the foundations of in situ concrete. The work examined three main areas within the design process. The first was to model the concrete elements then to model the reinforcing and ultimately production of the drawings. Because Autodesk Revit was the existing modeling program in the Department conducted the evaluation as a comparison between the two programs.
Optimization of immunoassay parameters in multiplex in the high throughput protein detection technique Proximity Extension Assay
The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.
Identifiering och kvalitetsbedömning av mineral genom Ramanspektroskopi
In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.Vi föreslår att man gör följande saker:Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttetUtvecklar och implementerar den föreslagna klassificerarenGenomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering.