The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

From embankment dams is something that has long been noticed and given rise to different questions. The most serious question is if the sludge is a result of inner erosion through the dam, and thus consists of material transported from the dam. Concerns have also been raised whether the sludge could interfere with the systems drainage capacity and thereby lead to high pore pressure in the dam, or if the measurement of seepage is disturbed, as the measuring is usually made via Thomson weirs, which could risk being blocked. The result of this investigation shows that the sludge is a result of the oxidation of iron by iron bacteria, and to the greater part consists of these oxides as well as the bacteria?s own structures. The iron oxidizing bacteria, FeOB, oxides ferrous iron to ferric as a part of their metabolism, and thereby create large quantities of iron(oxy)hydroxides.

Four different kinds of filter-materials with reactive surfaces have been studied concerning their capacity to absorb heavy metals in leachate from a municipal waste deposit. The heavy metals studied were: lead, cadmium, copper, mercury, chromium, nickel and zinc. The leachate contains high levels of dissolved organic carbon (DOC) and has a high pH-value along with a high buffer capacity. These characteristics of the leachate make it difficult to remove pollutants and require efficient filters. The filters that were examined in the report are blast-furnace slag with CaO, sand covered with iron oxides, olivine and nepheline.

It is estimated that 22 000 contaminated areas exist in Sweden today. In order to classify the risk of contaminant migration from these areas and to prioritize which of these contaminated areas should first be treated, the Swedish Environmental Protection Agency (EPA) has designed a method, for the investigation of contaminated areas (MIFO). One of these contaminated areas is the landfill Gräsötippen outside Köping. The landfill has been used by both Yara AB and the Köping municipality between the years 1945 and 1975, and both industrial and household waste have been deposited there. The aim of this report is to survey which contaminants that can be found in the soil and water in the landfill Gräsötippen and to evaluate the migration of these contaminants.

This master thesis has been a pilot study preceding a forthcoming project of a larger scale with the long term objective to separate the direct effect of added nitrogen on soil processes from indirect effects via trees. The aim of this study has been to investigate the allocation of nitrogen following direct injection of liquid solutions into the xylem of 40 year old Norway spruce. The field site is located at Flakaliden (64°07?N, 19°27?E), approximately 60 km northwest of Umeå, Sweden. A total of 18 trees were selected for treatment, equally divided between three treatments, potassium nitrate, glutamine and water (control).

SammanfattningSyfte: Finna forskningsbaserade underlag för hur fysisk barn- och ungdomsträning bör bedrivas, från nybörjarnivå som barn (ca sex år) till avancerad nivå som ung senior (ca 18 år) och koppla detta till innebandy.Frågeställningar: Finns det perioder under uppväxten då unga idrottare är extra mottagliga för en viss typ av träning? Hur bör träningen utformas under uppväxten uppdelat i delkapaciteterna: aerobt, anaerobt, styrka, rörlighet och koordination/teknik?Metod: Systematisk litteraturöversikt av befintlig forskning inom det valda problemområdet. Sökningar i databasen EBSCOhost har utförts. Från sammanlagt i 713 st. artiklar har tolv artiklar sållats fram som gick vidare till dataextraktion.

Cellulose nanocrystals (CNC) are great candidates for composite materials. The reasons why CNCs are such attractive materials for them are due to their great mechanical properties, high aspect ratio and low density. On the other hand, hydroxyapatite (HAp) is a kind of calcium phosphate and a main component of bones and teeth. The purpose of the present study is to make oriented nano-sized composites with CNC and HAp. Although some researchers carried out to make CNC composites with HAp for biomedical materials, nano-sized and oriented ones haven?t been achieved yet.

Important issues for the local authorities are what to do with the sludge produced insewagetreatment plants. The tax for landfill leads to higher costs with 250 SEK/ton. On the 31December 2004 it will be forbidden to landfill sludge.In Sweden the yearly production of sludge is 240 000 tons, counted as DS. The phosphorouscontent is 3 % in sludge. The total yearly production of sludge gives 6000 tons of phosphorous.

Fruit flies (Tephritidae) are serious pests that cause enormous losses for farmers in many countries. The frequent use of insecticides in controlling fruit flies in fruits and vegetable has not resulted in sustainable management of the pest. Problems associated with this complete reliance on chemical control are many residues of insecticides in crops, health problems for farmers, contamination of water and soil, insecticide resistance development and decrease in natural enemy populations. The implementation of control measures that do not imply an added burden to the environment and/or the farmers is urgent. More knowledge about the ecology of fruit flies is the basis for finding new and better ways to control this pest.

Brominated flame retardants (BFRs) has for many years been used in products to reduce their flammability, mainly in electronic products, textiles and construction materials. In 2003, Sweden imported 300 tons of brominated flame retardants. Leakage of these compounds has polluted natural environments. Fishes has shown increased contents of these substances, especially fat fish, since brominated flame retardants tends to accumulate in fatty tissues. They are also regarded as persistent and that gives them the ability to travel long distances. What also is really scary is that increased levels of brominated flame retardants have been detected in human breast milk. The knowledge of the brominated flame retardants is limited and not so much research has been done in this field. There are many reasons though, to keep the research going. Partly their structural resemblance to well-known toxics as PCB, but also their ability to accumulate in biological systems and enrich in food chains. In which way they affect humans we really don?t know yet.

This report was written by Sofia Ericson and describes the degree thesis with the title: ?Connections between the growing site of bio fuel, the chemical composition of bio fuel ash and the deposit growth in a bio fuel fired boiler.? The purpose of this degree thesis was to avoid melted deposit growth in the bio fuel fired boiler at ENA Kraft in Enköping. Potassium and sodium are known to lower the melting point of bio fuel ashes and are therefore not wanted in the trees in higher amounts than necessary for the growth. Silicon is also known to be important for the deposit growth but it is not possible to say in general if higher or lower contents of silicon is better. The relation between silicon and other chemical substances is more important than the total amount of silicon.

The RoHS Directive was introduced in order to restrict hazardous substances in Electrical and Electronic Equipment, EEE. It currently restricts the use of six hazardous substances/compounds; cadmium, lead, mercury, hexavalent chromium, PBB, and PBDE. The RoHS Directive currently includes category 1-7 and 10 in the categories of EEE listed in Annex 1A to the WEEE-Directive (Waste of EEE). The aim with the report is to investigate and elucidate prospects and obstacles to increase the scope of RoHS. This report mainly considers the inclusion of product categories 8 (Medical Devices) and 9 (Monitoring and Control Instruments).

Denna rapport är en studie där tre olika reningstekniker för att reducera utsläppen av VOC, (volatile organic compounds) på färgföretaget Beckers Group, jämförs och utvärderas. De reduceringsmetoder Beckers använder sig av i dagsläget vilka ska jämföras är på anläggningen i Sverige en uppkoncentrering med en zeolitrotor följt av katalytisk förbränning. Anläggningarna i Kina har ett skrubbertorn med diesel följt av en aktiv kolbädd som polersteg. Anläggningarna i USA har en katalytisk förbränningsmetod kallad Oxidizer.Teknikerna jämförs och utvärderas utifrån teknik, miljö och ekonomi i rapporten. Rapporten inleds med att teori gällande färgtillverkning, lösningsmedel och reningsteknikerna redogörs.

At the end of August 1999, the local government council of Malmö city decided thatthe area known as Kvarteret Bilen would be made available for the European HousingExhibition 2001 (Bo01-mässan). Kvarteret Bilen extends over approximately 80hectars and includes the western parts of the Western Harbour area (Västra hamnen)of Malmö.Kvarteret Bilen as a whole, but especially the western sections of this area, the socalledBo01 section, has been the subject of heated discussions. This is mainly due tothe fact that the ground in question has proven to be contaminated to a certain extentby different chemical compounds. Investigations undertaken during 1998 showed sporadically high concentrations forespecially one chemical group: polycyclic aromatic hydrocarbons (PAH).The first part of the paper describes the use of a mathematical equation (Fetter 1994)to calculate the concentration of PAH from a continuous discharge at a given time byemploying Geographical Information System (GIS). Even the constraints of theequation are discussed, as well as the consequences that follow as a result of usingGIS.The second part of the paper focuses on the connections between the content ofcarcinogenic PAH in Kvarteret Bilen and the ground water level and type of fillingmaterial.

Acid sulphate soils is a wide spread problem along the northern coast of Sweden. This is causing great damage through low pH and leaching of metals out into the streams. These soils are estimated to be found in several places in Sweden but also in many other areas in the world. One of these areas in Sweden that are believed to contain these sediments is Mälardalen. The purpose of this study is to evaluate the extension of the acid sulphate soils in this area.