Glutathione (GSH) is a molecule that consists of three amino acids: glutamic acid, cysteine and glycine. GSH has several important functions: to protect cells from free radicals, reactive oxygen species and oxidative stress. GSH exists in a reduced form, GSH, and in an oxidized dimeric form, glutationdisulfid, GSSG. The enzymes glutathionereductase (GR) catalyses the reduction of GSSG back to GSH. Nicotinamide adenine dinucleotide phosphate (NADPH) is required as a coenzyme in the reaction.

Most of the beef production in Sweden has been integrated with milk production. Since the 1990:s, the number of dairy cows has decreased. A specialised beef production has developed in purpose to compensate for this decrease. The consumption of beef have increased in the last decade, but the Swedish production has no followed this trend. In 2004, the percentage of beef self-sufficiency in Sweden was 60 %.

Ultrasound is still a relatively new method for the diagnosis of abdominal conditions in cattle. The objective of this study is to evaluate ultrasound as a diagnostic tool for the common diseases affecting high-producing dairy cattle, for example traumatic reticuloperitonitis, right- and left displacement of the abomasum, fatty liver, wound infections and abscesses. This paper is a combined litterature study and a report of the results from ultrasound examinations of bovine patients at the ruminant clinic at the Swedish University of Agricultural Sciences. The study includes two healthy cows from the ruminant clinic, and seven patients with abdominal related diseases. The examinations were made with a 2-6 MHz curvilinear transducer and both the right and left sides of the abdomen were scanned.

Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.

Nanotechnology, one of the most interesting sciences today will no doubt have an enourmous impact on tomorrow's society and industry. One of the most interesting nanomaterial that has been discovered is the semiconductor quantum dot (QD). This material shows interesting novel properties such as a size-dependent band gap, which is an effect caused by the quantum confinement effect in all three dimensions. Due to this phenomenon the absorption spectrum can be tuned to the desired value by adjusting the size of the QD.This thesis aims to investigate how the solvent ratio of H2O/Ethanol affects the optical and structural properties of copper sulfide QDs manufactured in a chemical synthesis way using modern microwave heating. The process of manufacturing QDs using chemical synthesis often involve a capping-agent to prevent the QDs from agglomerate into bigger structures.

The soybean can be used in multiple ways, and its high oil content makes it suitable for extraction. Soy bean meal, a by-product of the oil extraction, is a good source of protein with a high feeding value, and is therefore used in a number of different animal feeds. Further, soy bean meal has a low production price, which is one of the reasons for the extensive export of the product. There is a great demand for vegetable-based proteins, especially in the organic production where synthetic amino acids are banned. However, the current production of soy beans is not environmentally sound and that combined with the long transport distances amplifies the need of finding a locally produced protein source.

Mussel meal is a potential source of protein, with a good composition of amino acids, for laying hens and broiler chickens, and may replace the use of fish meal in organic poultry production. Lead and cadmium are heavy metals which occur in the environment and can be accumulated in mussels. When poultry are exposed to heavy metals, the metals are absorbed in the small intestine and distributed in the body in the form of ions, complexes or bound to proteins. In poultry, lead accumulates in bone tissue, liver and kidneys, while cadmium accumulates in liver and kidneys. Generally very low concentrations of these heavy metals occur in muscles of broilers which have been exposed to feed contaminated with cadmium or lead.

Abstract Tripeptidyl-peptidase II (TPPII), is present in most eukaryotic cells. It cuts tripeptides from the N-terminus of peptides and is especially important for degrading peptides longer than 15 amino acids. TPPII also tailors long peptides into suitable substrates for the enzymes which transport and produce the peptides that MHC I present. Increased levels of TPPII have also been found in certain cancer cells, thus it is of interest to determine if TPPII could be used as a tumour marker.The aim of this study was to optimise and evaluate 3 different methods for quantifying TPPII. Western blot, enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions.

The dairy cow?s ability to resume estrus after calving and to conceive is affected by their metabolic status after calving. For high yielding cows there is a high risk that the feed intake will not be sufficient to cope with the increased energy demands after calving, and the cow will end up in a state of negative energy balance. Energy balance can be measured in various ways, for example by body condition score or by plasma concentration of several metabolic hormones and metabolites. After calving, endocrine pathways stimulate tissue mobilization; insulin signaling is blocked, the concentration of insulin-like growth factor-1(IGF-1) and leptin decreases, and the concentration of growth hormone (GH) increases.

Det kommer ständigt nya studier med olika hälsoråd angående kosten och fysiskaktivitet om hur vi ska undvika fetma, högt blodtryck samt hjärt- och kärlsjukdomar.Intresset av proteinets effekt på uppbyggnad av muskelmassa vid fysisk aktivitet harökat. Detta har medfört ett stort urval av olika drycker med proteintillskott påmarknaden, främst ur animaliskt ursprung.Syftet med detta examensarbete var att ta fram en vegetabilisk proteinberikadhavredryck som förväntas hjälpa kroppen vid återhämtning. Arbetet innebar att utvecklaett recept baserat på de Nordiska Näringsrekommendationer 2004 (NNR) med fokus påprotein, kolhydrater och fett. Delmålet var även att utesluta allergener. Dryckenbaserades på ett medelvärde från de fysiskt aktiva och äldre människors näringsbehov,detta för att bredda den tilltänkta dryckens målgrupp.Fyra smaksatta drycker framställdes med olika energiinnehåll utifrån två olikahavredrycker, en naturell och en kalciumberikad.

The probiotic bacteria Lactobacillus reuteri DSM17938 was evaluated as a starter culture in cheese production, with the intention to produce a new interesting product within the functional food market. Growth and acidification rate in milk subjected to different heat treatments, as well as supplementation with growth promoting factors or support cultures was measured during controlled fermentations. The results showed that the weak proteolytic activity of L. reuteri could be compensated for by supplementation with 1% casamino acids or 5% MRS broth. Furthermore, a decrease in growth and acidification rate could be seen when using yoghurt culture or Lactobacillus delbrueckii as adjunct cultures.

AbstractAn approaching overproduction of cereals and a future uncertain supply of non genetic modified vegetable protein feedstuffs have increased the interest in an expansion of the domestic production of protein feedstuffs in Norway. Besides rapeseed (Brassica campestris), peas (Pisum sativum) may be the most suitable crop for this purpose. Peas are characterized by having a relatively high content of crude protein, ranging between 20-26%, a high content of starch, 42-51%, and a low content of fat. In general, the energy value for peas is higher than for barley but lower than for rapeseed and soybean meal.Pea protein consists of albumins and globulins to 85-100%, which leads to the fact that a large part of the pea protein is soluble and degradable in the rumen. Starch in peas is on the other hand to a large extent resistant to rumen degradation compared to starch from other starch rich feedstuffs.

The ammonia emissions are an environmental issue since they contribute to eutrophication. By far the largest source of ammonia emissions is manure from agriculture and should therefore be reduced. Loss of ammonia is not only negative for the environment but also to the farmer who loses nitrogen that could have been used by the crop. The focus of this study has been emissions from spreading slurry where the loss can vary widely depending on slurry-, soil-, and weather conditions. There are mainly two different ways to reduce the emissions. One is to inject or mix the slurry into the ground to avoid air contact. The other way is to use acid to reduce the pH in the slurry to a level between 5 and 6 where the equilibrium is so strongly pushed to NH4+ that hardly any NH3 can be lost.

Angelica archangelica (Garden angelica) is the only Medicinal and Aromatic Plant (MAP) with a Nordic origin. The plant can reach up to three meters when cultivated. Angelica archangelica is used as flavouring in additives, honey, beverage base, essential oils, fol-klore medicine and as ornamental for decorative purposes. Commercial cultivation is mainly focused on root production. Production countries are Poland, Netherland, France, Belgium, Switzerland, and former Czechoslovakia with an overall yearly world production of 1000 kg of essential oils.

Att rena vatten är kostsamt, från både en ekonomisk och miljömässig synpunkt, då behovet av fällningskemikalier, kolkällor och energi är stort. Det är därför önskvärt att titta på alternativa lösningar som möjliggör reningsverk att bli mer självförsörjande och kretsloppsanpassande.Hammarby Sjöstadverket är en pilotanläggning för avloppsvattenrening belägen i Stockholms sydöstra stadsdel i Henriksdal och ägs av IVL, Svenska Miljöinstitutet samt KTH. Sedan pilotanläggning byggdes år 2002, har flera olika reningstekniker utvärderats där fokus ligger på att sträva efter miljövänliga och kretsloppsanpassade system. Bland annat har en förfällningsteknik, så kallad trepunktsfällning utvärderats. Trepunktsfällningen innebär att ett metallsalt följt av två olika polymer tillsätts i flockningskammaren i inbördes ordning för att på så sätt kunna reducera en högre halt av det organiska materialet.