En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

The bacterium Lactobacillus reuteri, which natural habitat is in the intestine of mammals and birds, uses glycerol as a substrate for production of the antimicrobial compound reuterin. Glycerol has been shown to decrease the number of Escherichia coli in human feces and it is believed to be due to in situ production of reuterin. E. coli belongs to the large family of Enterobacteriaceae, naturally occurring in the intestine, and pathogenic strains of E. coli have been shown to be one of several bacteria causing diarrhoea in calves.

In southern Sweden, beech (Fagus silvatica) and spruce (Picea abies) are two species with similar demands on soil properties. With increased global warming and high acid deposition the future may bring larger areas were the two species are overlapping, competing for the same soil. The aim of this study was to compare spruce and beech concerning distribution, cultivation and soil properties. From available data comparisons were made between spruce and beech forests in Sweden. Soil analyses were made on samples from beech forest in southern Sweden, with and without natural regeneration of spruce.

Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.

Hundreds of thousands of piglets are castrated without anesthesia in Sweden every year so that we may be guaranteed meat without boar odor. The smell appears while cooking meat of boar and is described as urine- and sweat-like. High levels of androstenone and skatole are considered the main factors behind boar odor. Skatole is a metabolite of the amino acid tryptophan, which is formed in the colon of all pigs. Androstenone is a steroid hormone that is released from the Leydig cells in the testes of boars.

Syftet med arbetet var att sammanställa vad som tidigare har skrivits om de essentiella fettsyrorna och huruvida de har effekt på ögats främre delar, framför allt på det torra ögat.Denna litteraturstudie har genomförts med hjälp av artiklar som hittats på PubMed och Google Scholar, med hjälp av sökord såsom ?nutrition, dry eye, omega-3 och Fatty acids?,men även med hjälp av andra artiklars referenslistor. Artiklarna har sedan sammanställts tilldetta arbete.Efter sammanställning av artiklarna är det möjligt att skönja en mildrande effekt av omega-6 fettsyror på symtom på torra ögon och även en ökning av tårproduktionen. Även omega-3 fettsyror tycks ha dessa egenskaper och kan därför hjälpa det torra ögat. Dock tycks en högre omega-6:omega-3 ? kvot leda till en ökad risk för torra ögon, vilket faktiskt till viss del motsäger resultatet att omega-6 fettsyror fungerar förbättrande för torra ögon.

It is possible to affect the feed costs in different ways. By steeping or fermentation the digestibility of the feed is improved for the animals with a natural process in the wet feeding system Steeping means that the feed is soaked some hours before feeding. This process activatesenzymes and improves the digestibility of for example phosphorus in the feed and make it more accessible to the animal. Wet feeding can be fermented or not. Fermentationmeans that the feed has been soaked for a certain length of time so that a biological process is started.

This thesis raises the problem with acid mine drainage around the closed mine Finngruvan.The purpose of the investigation was to compare metal content in surface water in the areaand its catchments between year 2010 and 2003, also to make calculation of specific runoffand metal load, furthermore determine the recipients in the area. Surface water samplingwhere preformed at the same place as 2003 and also at a few other places of interest. Adigital elevation file were used in GIS to calculate the area of the watersheds in the region,observations in the field where also made. The collected data were used to make comparisons,calculations and conclusions. The result showed that the surface water 2010 had somewhatlower metal concentrations.

This report describes a new foundation method and compares it with a traditional founding of concrete. This new method is called the Koljern technique and uses cellular glass which is called Foamglas. The Foamglas is compounded to founding elements by lightweight steel beams. Foamglas is an insulation material with high compressive strength, and is rot, insect, vermin and acid proof. The cellular glass is also fire proof and watertight, which makes it a very good construction material.The report contains a study of a number of cases for which the Koljern technique is compared with a foundation of concrete.

Sammansättningen och kvalitén på mjölken som produceras är betalningsgrundande och därmed är det viktigt ur en ekonomisk synvinkel att producera mjölk som håller en hög standard. Syftet med denna litteraturstudie var att undersöka om samband finns mellan typ av digivning respektive avvänjning och mjölkens sammansättning. Fokus ligger på mjölkfettet samt fettsyrorna. De produktionssystem som tas upp är begränsad digivning och artificiell uppfödning. Begränsad digivning innebär att avkomman får gå med moderdjuret vissa timmar av dygnet fram till en senare avvänjning jämfört med artificiell uppfödning där avkomman skiljs från moderdjuret strax efter förlossningen.

Glycerol is essential for the lipid metabolism of both plants and animals. Glycerol is formed in the rumen by hydrolysis of lipids from the feed. Glycerol is also a by-product from the production of biodiesel from rapeseed oil and other fat sources. Glycerol might be used as a dietary glucose precursor for dairy cows in similar ways as propylene glycol. Due to increased production of biodiesel more glycerol has been available to a lower price.

In the year 1783 a fissure eruption from Laki in the southern part of Iceland produced a large amount of volcanic gases during a period of eight months. The volcanic gases and aerosols spread across the northern hem sphere due to ideal weather conditions and had a substantial impact on the environment, human health and also climate effects. This, so called dry fog, caused severe health problems, which led to death casualties, killing animals, crops and other vegetation. The consequence was a widespread famine which was aggravated by a hard and long winter in 1783/84. Analysis shows that the Icelandic volcanoes are the primary risk to produce dry fog that can affect Sweden.

The mining industry, in terms of ore excavation and metal production, is and has been important to the Swedish economy. In connection with the ore excavation process, large amounts of partly sulphidic waste are produced. When the metal sulphides are exposed to air and water they are eventually oxidised, which leads to the formation of acidic water with high concentrations of metal cations and sulphate ions, so called Acid Mine Drainage (AMD). This water may reach the nearest surroundings and cause negative environmental effects. By covering the waste with soil or water, the oxidation process can be minimised. At the Rävlidmyran mine in the county of Västerbotten open pit mining took place between 1951 and 1991.

During the last decades the fertility of dairy cows has declined in the same rate as the milk yield has increased. This is a result of the negative genetic correlation between milk yield and fertility, failure to show estrous signs and metabolic problems in the cow. The purpose of this review was to investigate the function of metabolites and hormones as markers for disturbances in fertility, health and production in the dairy cow. There are several metabolites that are suggested to indicate the cow?s energy balance.