Syfte med studien var att undersöka pedagogernas uppfattning om arbetet med hållbar utveckling genom en Grön Flagg-certifiering i förskolan. Mina frågeställningar blev därför: Hur stödjer en Grön Flagg-certifieringen arbetet mot hållbar utveckling? Hur görs barnen delaktiga i arbetet med Grön Flagg? Hur ser pedagogerna på sig själva som förebilder? Dessa frågor har besvarats genom en undersökning med kvalitativa intervjuer av åtta pedagoger i fyra olika Grön Flagg-certifierade förskolor (bland dessa en förskoleadministratör) samt kommunens miljöstrateg, tre barns egna tankar om miljö och yngre kamrater har undersöks.  Resultat har analyserats utifrån lärande för hållbar utveckling, Harts delaktighetstrappa samt Axness fostrans principer utifrån ett nytt forskningsområde kallad Epigenetik med fokus på ett av dess användningsområden: barnuppfostran. Genom resultat och analys av datainsamlingen har jag funnit att en Grön Flagg-certifieringen kan stödja arbetet mot hållbar utveckling om det finns ett gemensamt synsätt inom arbetslaget, engagerade och drivande pedagoger samtidigt som man synliggör de små vardagliga insatserna i förskolan. Pedagogerna i studien anser att certifieringen utåt visat att man arbetat mot hållbar utveckling och sätter press till att arbetet genomförs.

Mammary tumors are the most frequent type of tumors in bitches in Sweden. Many studies have been published concerning mammary tumors, but there is still much to be learned about this disease. One has, since some time ago, had the knowledge that addition to or removal of methyl groups to genes can inhibit or activate gene-expression. Methylation and demethylation is a normal phenomenon in the body and it is essential for the development and survival of the individual. The risk of developing a tumor disease is increased if the global methylationstatus is changed; if oncogenes demethylates (activates) or if tumor suppressor genes methylates (inactivates). In the human medicine one has seen that there are differences in methylationstatus between different tumors. In this study tumor and normal tissue from ten dogs were examined with regard to methylationstatus.

The dairy cow?s ability to resume estrus after calving and to conceive is affected by their metabolic status after calving. For high yielding cows there is a high risk that the feed intake will not be sufficient to cope with the increased energy demands after calving, and the cow will end up in a state of negative energy balance. Energy balance can be measured in various ways, for example by body condition score or by plasma concentration of several metabolic hormones and metabolites. After calving, endocrine pathways stimulate tissue mobilization; insulin signaling is blocked, the concentration of insulin-like growth factor-1(IGF-1) and leptin decreases, and the concentration of growth hormone (GH) increases.

ABSTRACTThe aim of this study was to give an overview of the evidence for genomic imprinting in livestock and other mammals as well as outline the potential use of gene imprinting in livestock breeding. Epigenetics is the mitotical and meiotical partial hereditary variation in genomic activity without any alterations of the DNA sequence. An example of epigenetic regulation is genomic imprinting where one allele?s expression differs depending on which parent it was inherited from. These parent-of-origin effects are currently overlooked in livestock production.

This study was conducted at the University of Bath, UK, from September to December 2014 as part of an ongoing research project aimed at elucidating how the Grb10 gene might act as a mediator of long-term health effects (such as predisposition to obesity, diabetes, coronary heart disease and hypertension) caused by environmental factors during development. This phenomenon is known as developmental programming. The purpose of this thesis was not primarily to answer any of the broader questions posed by this research project at large since this would require much more data than is reasonable to acquire over the course of a few months, but rather to evaluate the methods used in this project and reveal whether they are working the way they are presumed to. In the research project, which employs mice with the Grb10 gene knocked out as well as wild-type control mice, a dietary restriction model is utilized during gestation, which is supposed to generate offspring with lower birth weight and subsequent detrimental health effects in adulthood. This is supposed to be achieved by restricting the protein content of the pregnant mothers? diet to 9 % (as opposed to the control diet of 20 %) throughout gestation.

Igf2 (insulin-lik tillväxtfaktor 2) och H19 är präglade gener hos däggdjur. Präglade gener uttrycks ojämnt; den allel som ärvts från den ena föräldern uttrycks mer än den från den andra. Igf2-genen kodar för en viktig embryonal tillväxtfaktor och uttrycks till protein bara från den paternella allelen. H19 transkriberas bara från den maternella allelen, men translateras inte till protein. Igf2 spelar en viktig roll under dräktigheten, då den bidrar till tillväxten av både placenta och foster och reglerar såväl näringstillgång och tillväxthastighet för embryot. Enligt en teori kallad ?the genetic conflict theory? (~konflikten mellan genomen) så ligger det i honans intresse att fördela resurserna jämnt mellan alla syskon.