Nuts of all kinds are common ingredients in food. For nut allergy sufferers the frequent use of nuts cause problems and "hidden" nuts in food products may elicit allergic reaction when such foods are consumed. Methods for detecting and quantifying walnut (and other nuts) with high sensitivity and specificity are therefore very important.The objective of this project was to verify the specificity of a rabbit antiserum against walnut with immunodiffusion and to determine the size of the dominant walnut antigens with Western blotting. In addition, a commercial sandwich ELISA for walnut quantification was validated and compared with a qualitative real-time PCR.The rabbit antiserum proved to be less specific but after absorption with cross-reacting nuts and seeds it showed high specificity. The ELISA kit reacted, except for walnut, with pecan and slightly with other nuts and seeds tested.

AbstractThe aim of the studie was to investigate if the Pediatric Early Warning Score system, PEWS, is an useful instrument on a children's inpatient ward, regarding the detecting of deterioration in children's vital signs. For this, a quantitative descriptive design with qualitative elements was used. Method: Study specific questionnaires were distributed to 86 persons (helpnurses, nurses and doctors). Of these, 36 answered and sent back the surveys. Results: Of 36 responses 25 felt experienced a faster Detection of deterioration of vital signs in the child.

During the last ten years the cultivation of maize in Sweden has increased and is expected to grow further. Most of the maize in Sweden becomes silage which is used to feed animals at farms. Maize has in other countries been shown to be a substrate for growth of mould and especially Aspergillus spp., Fusarium spp. and Pencillium spp. has been reported.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

Early intervention is important on different levels and must be justified as a social and economic long-term investment. There is no doubt about the importance of early Detection when helping children/students in need of support. What kind of support that is deemed suitable and who provides the support can be viewed from different perspectives and with different approaches. Seen from a relational perspective the special education process is a long-term process and affects not only the individual but the whole school culture and systems may need changing. This study intends to describe the importance of the learning environment and what efforts are initiated early for students in need of support from the special education point of view.

This master thesis reviews advances in Detection and diagnosis of static friction (stiction) in control valves. Control valves suffering from stiction results in oscillating control loops which in turn decreases both the process stability and reliability. Perstorp AB sees a necessity to automatically detect stiction in valves before the effects of the malfunctioning valves have become too severe. This thesis presents an introduction to maintenance, control loops and how stiction in valves can affect performance of control loops.A selection of methods presented in the literature are introduced and examined using normal operating data from the site in Perstorp. Common for all selected methods is their intuitive ideas and that they are based on normal operating data and minimum amount of process knowledge.

White-Nose Syndrome, WNS, is an emerging fungal disease in the Northern American bat population causing mass mortality in infected hibernacula. Geomyces destructans is a newly discovered psychrophilic fungus causing WNS but the pathogenesis and epidemiology of the disease is not yet fully clarified. Geomyces destructans has been found in Europe but has not been a cause of mass mortality there. This study is the first investigation done to see if G. destructans is present in Sweden.

Several factors affect the heifer´s growth and development, the newborn calf´s feeding of co-lostrum during its first hours of life is one important factor. The colostrum contains higher amount of immunoglobulins and insulin growth factors compared to matured milk. A high intake of immunoglobulins from colostrum results in a high blood concentration of immuno-globulins until the age of eight months. This leads to a lower incidence of diseases that can affect the growth and development negatively. Diarrhea is the most common disease in calves younger than 30 days.

Tolkningen av EKG är en viktig metod vid diagnostisering av onormala hjärttillstånd och kan användas i förebyggande syfte att upptäcka tidigare okända hjärtproblem. Att enkelt kunna mäta sitt EKG och få det analyserat och presenterat på ett pedagogiskt sätt utan att behöva rådfråga en läkare är något det finns ett konsumentbehov av.Denna rapport beskriver hur en EKG-signal behandlas med olika algoritmer och metoder i syfte att detektera hjärtslag och dess olika parametrar. Denna information används till att klassificera varje hjärtslag för sig och därmed avgöra om användaren har en normal eller onormal hjärtfunktion. För att nå dit har en mjukvaruprototyp utvecklats där algoritmerna implementerats. En enkätundersökning gjordes i syfte att undersöka hur utdata från mjukvaruprototypen skulle presenteras för en vanlig användare utan medicinsk utbildning.Sju filer med EKG-signaler från MIT-BIH Arrhythmia Database användes för testning av mjukvaruprototypen.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

NMD 100 is a notes and media dispensing machine, manufactured by De La Rue Ltd, located in Flen, Sweden. The customers, mainly bank offices, have expressed a need for a more comprehensive description of errors occurring from time to time in their machines. In order to acquire a BSc-degree in Computer and Electronics Technology at the University of Linköping I have carried out a diploma work, regarding new computer programs to fulfil the customers? wishes. The machine NMD 100 is connected to its PC through handshaking.

There are several objects of age whose provenance and history has disappeared with time. The purpose of this thesis is to find out which analyzing methods that gives results and how the different methods can be combined and /or established in each other. The investigations centers around an embroidery, belonging to Tämta church, with unknown origin, use and age. The embroidery is long and narrow with applications in the form of dragons and eagles on a ground of velvet cloth. The dragons and eagles are covered with metal threads.

Haptoglobin is an acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years.    Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The Detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed.    Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.

This master's thesis is a study of how librarians are depicted in crime fiction. 12 American and English mysterynovels featuring librarians in a central role were studied and analysed using character theory. Recurring traitswere identified and organised into themes. A number of prominent traits and themes emerged that show librariansas orderly and organised bibliophiles, but with a taste for adventure and excitement. They are keen problem solverswho enjoy a challenge, at work, or in the form of crime Detection.

Traditionally, rolling mills use emulsions based on a mixture of oil and water for lubrication. Since two years ago SAPA has been using (instead of oil) a synthetic lubricant so called conditional emulsion for hot-rolling of aluminum. This lubricant is water based and homogenous at ambient temperature, but switches to a two-phase system at heating above the cloud point.This project aims to validate and if necessary modify an existing HPLC method for quantifying two out of three key components (A, B and C) in the conditional emulsion. Attempts to develop a method to quantify the pH adjusting components, X and Y were also made. These two methods are required to optimize the lubricant.Due to the complexity of the components, it has been difficult to present a method for quantification, and HPLC with ELS Detection was chosen after a long series of trials.