Urbanization has for a long time been a threat to our biodiversity. Epiphytic organisms such as lichens have been negative affected by traffic pollution and the new urban environment surrounding their old habitat (van Her 2001). Another epiphytic organism is the green algae Desmococcus olivaceus. Unlike lichens the green algae is favoured by polluted areas. The aim of this study was to investigate if time in urban environments affected the cover of the green algae on old oaks.