Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

This work was conducted during spring and summer 2012 together with the company Uppsala Academy of Management as the principal. The two main objectives for the work are that: ? Make a comparison between the measured volume in the standing forest and the measured volume at the industry on Uppsala Academy of Managements actual stumpage sale volume to see how well it conforms. ? Examine if the buyers see any particular advantages or disadvantages of actual stumpage sale volume and what they think of Uppsala Academy of Managements actual stumpage sale volume and structure in general. I chose to use actual stumpage sale volume from the tree years 2009, 2010 and 2011 in my work. I divided the actual stumpage sale volume following four categories which was pine, spruce, pine/spruce and seed trees. After that I picked out sixteen actual stumpage sale volumes from each category to be a part of the work.

In this literature review the cemetery is studied in its role as a public space in an urban context. To be able to scrutinize the cemetery?s relationship to the public domain in the future, the cemetery?s background and history is studied in the beginning of the essay. The cemetery?s connection to the church, as well as a variety of other factors such as economic, architecture, hygienic aspects and different ideals has influenced the cemetery to be the place it is today.

The purpose of this study is to examine the view of Swedish language teachers on the role of fiction and of school libraries within the compulsory school?s teaching of Swedish of form 6-9. The questions to be answered are:? What part do Swedish language teachers (hereafter called teachers) think fiction is playing regarding the pupils inclination to read and reading promotion?? What is the attitude of teachers regarding the function of fiction in the Swedish subject?? What do teachers consider being the role of the school library in education, and can this be related to the local school library resources?To answer the questions a questionnaire was sent out to thirty-five teachers. Four qualitative interviews were also carried out among the fourteen teachers that answered the questionnaire.

Beef cattle wintering outdoors should be provided a shelter, e.g. a type of barn or corresponding. This type of production requires appropriate soil types and secured animal welfare. For example, a clean and dry resting place needs to be provided to the animals. To receive an exemption for buildings the farmer has to have something equivalent that provides an adequate shelter.

Abortion remains a contentious issue, which greatly raises ethical problems. This thesis examines the leading arguments in the Swedish abortion debate around the ? in terms of legislation ? critical years of 1938 and 1974. With Foucault's genealogical method, Butler's theories of gender as a social construction in relation to power and doxa, the study examines what the development of the argumentation says about the views on morality, the woman's body, life, individual and state? How has the arguments pro and against abortion changed over time and what does this say about the norms that guide and orientate the arguments? The most obvious change in argumentation is seen in the shift from an absolute moral and conservative position into an increasingly relativistic and liberal position, and this study will argue that this shift has its prerequisites in the changing status of the citizen and state.

This Master Thesis has been carried out as a new product development project in collaborationwith G-Man Tools AB located in Edsbyn. The purpose of the project was to construct a newpruning saw there the blade is integrateble in the handle, thus a foldable pruning saw.The work has been performed on the occasion of G-Man Tools AB wanted to improve theirproduct range by creating a saw that complement their range of quality saws. They have a similarproduct today, but they do not manufacture this product by them selves and it is not directlytargeted against garden use, which is a disadvantage when it is one of their major markets.Even though almost the entire chain from the requirements of the users to drawings has beentaken into consideration, emphasizes has been put on generating ideas. However the first thingthat was carried out was to establish a productspecifikation from interviews with users and thecompany?s experience.

It is sixty years since Sweden's first star-shaped housing estate was built in Gröndal, a suburb of Stockholm. Olle Engkvist, the building contractor, was the driving force behind the residential area and it was designed by architects Sven Backström and Leif Reinius. The estate comprises detached star-shaped houses suited to a more hilly terrain and terraced star-shaped houses on the flat land. The eight gardens of the terraced houses form the shape of a hexagon. It is these gardens, designed by Sven Hermelin and Per Friberg, which are the main focus of this thesis. The buildings on the estate have recently been renovated and it is now time for the gardens to be restored.

Assessing and measuring post-operative pain in all animals is a challenge, but doing it in small mammals, for example guinea pigs, presents an even larger difficulty. Validated pain scales developed for use in dogs in clinical practice are currently available, but no such scale has been widely accepted for use in guinea pigs in the veterinary setting. In this experimental study, six guinea pigs that had undergone surgery and anaesthesia were observed for behavioural changes that could be associated with pain. The six guinea pigs were also given pain scores with the use of Colorado State University Feline Acute Pain Scale (CSU-APSF), a scale which uses both behaviour when not handled, body tension and response to palpation to evaluate pain intensity. The six guinea pigs showed a number of different behaviours, for example rigid posture, fixed stare and ?wet dog response? (the body is vigourosly shaken), that could be associated with pain.

Background: According to the Swedish Social Board induced abortions are increasing in the modern day society. Nurses who work with in the gynecological department can come across patients seeking care for an induced abortion.Theoretical framework: The findings of the study will be discussed in relation to Peplau's nursing theory, ethical concepts and nursing concepts.Aim: The aim of the literature review was to illuminate the experiences of nurses working with induced abortion.Method: A systematic literature review with an inductive approach was chosen. The findings are based on seven qualitative and two quantitative studies. The articles were assessed through two different modified templates based on qualitative or quantitative design.Findings: Nurses and midwives experienced their work with induced abortions as emotionally stressful. However, it was highlighted that all women have the right whether to choose abortion or to proceed with the pregnancy.

Libraries are often associated with positive values such as democracy and equal rights. The contents of the literature on offer should therefore be of great interest to the librarian, the supplier of said literature. The topic of this Master?s thesis is the popular literary genre known as chick lit. The purpose is to investigate the love theme in Swedish chick lit, and how it relates to Anthony Giddens? theory on love in late modernity.

The ability to tackle NP-hard problems has been greatly extended by the introduction of Metaheuristics (see Blum & Roli (2003)) for a summary of most Metaheuristics, general problem-independent optimisation algorithms extending the hill-climbing local search approach to escape local minima. One of these algorithms is Iterated Local Search (ILS) (Lourenco et al., 2002; Stützle, 1999a, p. 25ff), a recent easy to implement but powerful algorithm with results comparable or superior to other state-of-the-art methods for many combinatorial optimisation problems, among them the Traveling Salesman (TSP) and Quadratic Assignment Problem (QAP). ILS iteratively samples local minima by modifying the current local minimum and restartinga local search porcedure on this modified solution. This thesis will show how ILS can be implemented for MSA.

The ability to tackle NP-hard problems has been greatly extended by the introduction of Metaheuristics (see Blum & Roli (2003)) for a summary of most Metaheuristics, general problem-independent optimisation algorithms extending the hill-climbing local search approach to escape local minima. One of these algorithms is Iterated Local Search (ILS) (Lourenco et al., 2002; Stützle, 1999a, p. 25ff), a recent easy to implement but powerful algorithm with results comparable or superior to other state-of-the-art methods for many combinatorial optimisation problems, among them the Traveling Salesman (TSP) and Quadratic Assignment Problem (QAP). ILS iteratively samples local minima by modifying the current local minimum and restartinga local search porcedure on this modified solution. This thesis will show how ILS can be implemented for MSA.

In the government's bill on infrastructure for 2010-2021, the government presents that road investments should be prioritized before investments in the railway. The grants for operation and maintenance of the railways are much higher then in the last government's bill. Furthermore the current founding of investment in railways is higher then for road investment which is in opposite of the bill on infrastructure's directives. Therefore the purpose with my thesis is to find which factors that can explain how come the government has chosen to take these actions. As a model to help me achieve my purpose I have been using a modified version of the DBO-theory, where the basic factors to explain an action is Desire, Belief and Opportunities.

A lot of habitats have declined or disappeared as the old cultural landscape changed to the modern agricultural landscape of today. Places such as power line corridors, racing tracks, gravel pits and military training sites have become refuges for many species associated with the old cultural landscape. These sites have been exposed to ecological disturbances that create the same type of habitats that were found in the old cultural landscape.The military training sites in Sweden has an impressive diversity of species. Disturbances from the military exercises have created habitats that are important for a wide range of species.The purpose of this thesis is to study some of the ecological disturbances occurring in the military training sites of the Swedish Armed Forces and examine how they affect different types of habitats. The thesis explains through litterateur and field studies the reason to the rich biodiversity often found at military training sites.