The main objective of this study is to divide a number of colorectal cancer cases into subgroups based on their molecular features using cluster analysis. The data used is supplied by a research group at Pathology, the Department of Medical Biosciences, Umeå University, and consists, after some preparation, of 455 observations which is a larger data set than many similar studies. The molecular variables that the clustering is based on are CIMP (CpG Island Methylator Phenotype), MSI (Micro Satellite Instability), BRAF- and KRAS-mutations. These are categorical variables and consequently the clustering method used is PAM (Partitioning Around Medoids) which is particularly useful with data on diverse variable level. The final analysis results in four subgroups that are represented by different combinations of attributes on the aforementioned variables.

The main objective of this study is to divide a number of colorectal cancer cases into subgroups based on their molecular features using cluster analysis. The data used is supplied by a research group at Pathology, the Department of Medical Biosciences, Umeå University, and consists, after some preparation, of 455 observations which is a larger data set than many similar studies. The molecular variables that the clustering is based on are CIMP (CpG Island Methylator Phenotype), MSI (Micro Satellite Instability), BRAF- and KRAS-mutations. These are categorical variables and consequently the clustering method used is PAM (Partitioning Around Medoids) which is particularly useful with data on diverse variable level. The final analysis results in four subgroups that are represented by different combinations of attributes on the aforementioned variables.

The main objective of this study is to divide a number of colorectal cancer cases into subgroups based on their molecular features using cluster analysis. The data used is supplied by a research group at Pathology, the Department of Medical Biosciences, Umeå University, and consists, after some preparation, of 455 observations which is a larger data set than many similar studies. The molecular variables that the clustering is based on are CIMP (CpG Island Methylator Phenotype), MSI (Micro Satellite Instability), BRAF- and KRAS-mutations. These are categorical variables and consequently the clustering method used is PAM (Partitioning Around Medoids) which is particularly useful with data on diverse variable level. The final analysis results in four subgroups that are represented by different combinations of attributes on the aforementioned variables.

This literature study aims to examine the existence of cancer stem cells in breast cancer. The cancer stem cell theory states that there is a hierarchical organization within a tumour, in which a small subpopulation of the cells can initiate new tumours and maintain tumour growth whilst the bulk of the tumour cannot. These tumour initiating cells have shown to possess many characteristics similar to those of adult stem cells, which is why they are often referred to as cancer stem cells. Both cell types have the capacity of asymmetric division and have shown to possess mechanisms of resistance to both apoptosis and cancer drugs. The cancer stem cell theory elucidates many biological aspects such as the heterogeneity of tumours and the relapse of many cancers after what appeared to be successful treatments.

The main objective of this study is to divide a number of colorectal cancer cases into subgroups based on their molecular features using cluster analysis. The data used is supplied by a research group at Pathology, the Department of Medical Biosciences, Umeå University, and consists, after some preparation, of 455 observations which is a larger data set than many similar studies. The molecular variables that the clustering is based on are CIMP (CpG Island Methylator Phenotype), MSI (Micro Satellite Instability), BRAF- and KRAS-mutations. These are categorical variables and consequently the clustering method used is PAM (Partitioning Around Medoids) which is particularly useful with data on diverse variable level. The final analysis results in four subgroups that are represented by different combinations of attributes on the aforementioned variables.

Malign hjärntumör är en vanlig sjukdom som ger olika former av symtom beroende på var tumören är lokaliserad och behandlingsformerna kan ge olika komplikationer. Det finns inga tydliga riktlinjer för hur omvårdnaden kring patienten med malign hjärntumör ska bedrivas. Syftet med denna studie var därför att sammanställa ett kunskapsunderlag angående omvårdnadsåtgärder för patienter med malign hjärntumör. Metoden som användes var en litteraturstudie i form av en granskning av vetenskapliga artiklar, vårdprogram, kandidat- och magisteruppsatser och böcker. Resultatet visade vem, hur, var, vad och när information ska ges till patienten.

With increased milkproduction in our dairy cattle, comes a risk for a reduced fertility. Studies performed on animals and humans have shown that it is possible to manipulate the oestrous cycle through exposure to pheromones. This means that pheromones could potentionally be used to make reproduction more efficient. To evaluate the bioactivity of the substances in question, there is need for a bioassay. In insects, pheromones can induce a changed cardiac activity.

Today, some kind of laboratory results is required for around 70% of the diagnostics and follow-ups for diseases. In many of the cases the time from sampling to a result is very critical. Therefore the discussion of how to improve this situation has begun. For many analyses serum has been the routine choice for a long time but now it is disputed. After blood collection in a serum tube it is essential to wait 30-60 minutes before centrifugation and analysis of the sample, a long time for someone in an acute state.

Bakgrund: Varje år drabbas ungefär 1100 personer i Sverige av en hjärntumör. Symtomen som uppstod innan diagnosbesked upplevdes som mindre allvarliga, men under behandlingen blev symtomen svårare. Hjärntumören påverkade hela patientens livsvärld. Det var sjuksköterskans ansvar att stötta patienten och att hjälpa patienten att uppnå livskvalitet. Hoppet var ett existentiellt grundvillkor som hjälpte människor att skapa mening med livet.

Serum thymidine kinase 1 (TK1) activity is used as a tumor marker in disease monitoring in veterinary and human medicine. TK1, an intracellular enzyme, is involved in a salvage pathway of DNA precursor synthesis. TK1 is used in DNA precursor production by catalyzing the transfer of the gamma-phosphate-group from a phosphate-donor to the 5?- hydroxyl-group of thymidine forming thymidine-monophosphate. Nucleoside monophosphosphates are finally converted into thymidine-triphosphates. TK1 activity significantly rises in the G1 and the S phase of the cell cycle.

Breast cancer is the most common tumor disease among women in Sweden. About 7000persons, having a median age of 65, are diagnosed each year with this disease. Withmammography screening, breast cancer can be detected in an early stage which improves theoverall survival (OS). 20-30 % of the breast cancer tumors are overexpressing humanepidermal growth factor receptor 2 (HER2), which is a protein that stimulates cell proliferation.Trastuzumab (Herceptin®) is a humanised monoclonal antibody that targets the HER2-proteinand prevent the signals for cell proliferation.Trastuzumab has earlier been used for treatment of metastatic breast cancer. In the year of 2007trastuzumab was approved for adjuvant treatment of patients who has been medicated withsurgery and/or radiation.The aim of this study was to investigate the effects of adjuvant treatment with trastuzumab inHER2-positive breast cancer patients.

Tissue factor (TF), a 47 kDa glycoprotein, is the initiator of the extrinsic pathway of blood coagulation and consequently of the upmost importance when damage to blood vessel occurs. The source of TF in circulation has been investigated. However, the source of TF is still not clear. One theory is that platelets express and increases the expression of TF after stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation.Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardiac infarction were analyzed by using real-time polymerase chain reaction (PCR). Gel electrophoresis was performed after amplification of TF mRNA to verify the results.The samples were negative for TF when using real-time PCR and the few positive all had cycle threshold (Ct) values above 35.

Bakgrund: Trots allmänna råd och riktlinjer råder stor brist på nutritionskunskaperna inom hälso- och sjukvården i hela Europa. Många äldre riskerar att drabbas av undernäring och får ett minskat välbefinnande och en för tidig död. Genom att arbeta med att personanpassa kosten kan patienten få bli aktiv i egna beslut som påverkar dennes liv och hälsa. Syfte: Syftet med studien var att belysa omvårdnadspersonalens erfarenheter av kost som ges personanpassad på ett korttidsboende för äldre. Metod: I denna studie användes induktiv ansats där datamaterialet analyserades med kvalitativ innehållsanalys. Sex omvårdnadspersonal intervjuades på ett korttidsboende för äldre i södra Sverige. Resultat: Ur analysen av datamaterialet fyra kategorier; utgår från patientens syn på situationen, möjliggör val, möjligheter i måltidsmiljö, begränsningar i arbetsmiljön med åtta underkategorier.

Alla människor kan drabbas av hjärtstopp och varje år inträffar ungefär 15 000 hjärtstopp i Sverige. Att drabbas av hjärtstopp är en traumatisk upplevelse som påverkar individen på olika sätt. En ökad kunskap om patienters upplevelser utgör grunden för att ge professionell omvårdnad och kunna bemöta patienten i sin livsvärld. Syftet var att belysa patientens upplevelser efter att ha överlevt hjärtstopp. Studien genomfördes som en litteraturstudie där tio vetenskapliga artiklar utgjorde underlaget för resultatet.

The objectives of this study was to examine the prevalence of hypertrophic cardiomyopathy in a family of British Shorthaircats to determine the mode of inheritance and to investigate if the disease was associated with a mutation in myosin binding proteinC (MyBPC3). The family comprised 28 cats, seven male and 21 females. The cats underwent a physical examination, including cardiac auscultation, and an ultrasound examination. Blood was collected for DNA- analysis. The blodsamples were sent in a buffer solution to Kathryn Meurs, Washington State University, USA for analysis, where the genes coding for MyBPC, Troponin I and T were characterized using microsequencing technique. Out of the 28 cats, 8 were diagnosed with HCM, 2 were diagnosed with congenital heart disease but had no evidence of HCM.