Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

The demand for animal products has increased in the last decades, especially for poultry and pork. Pork production has increased drastically globally, and also in Uganda. Thirty-one percent of Uganda´s population lives in poverty. Pig production is believed to play a crucial role in poverty alleviation, because of the ability to convert low quality feed into high quality protein together with high reproductive potential. African swine fever (ASF) is a lethal disease of domestic pigs often characterized by haemorrhagic fever and with mortality rates up to 100%. The disease is endemic in most sub-Saharan countries and since 2007 in the Russian Federation and Caucasus area.

Vatten är ett livsmedel som vi kommer i kontakt med dagligen. För att inte råka ut för sjuk-domar och infektioner renas dricksvattnet på vattenverken, främst för att reducera antalet patogener, d.v.s. sjukdomsframkallande mikroorganismer. Man brukar prata om tre grupper mikroorganismer i vattenrening; bakterier, virus och parasiter. Dessa grupper är vitt skilda i många avseenden och reduceras därför olika bra av olika reningssteg.

I media kan man ständigt höra om ökande hot från virus och annan skadlig kod, samtidigt som botnäten bara blir större och större. Med fler och större hot på Internet så blir det allt viktigare att veta hur man kan försvara sig mot dessa. Denna rapport utvärderar en mängd verktyg som kan hjälpa till att hitta svagheter i nätverk, sedan rekommenderas en verktygslåda med de främsta av dessa. Slutligen så finns det en liten guide hur några av dessa verktyg kan användas tillsammans. De områden som främst behandlas är Nätverksmappning och Portscanning.

Background;Chronic obstructive pulmonary disease (COPD) is a lung disease characterized by airway obstruction. Common signs of COPD are the slow process and shortness of breath on exertion. The most important treatment is to quit smoking. Most nurses, regardless of where they work, will meet these patients, often when the patients are having respiratory disorders. Aim;the aim of this study was to illuminate how patients with COPD describe the daily life based on how he/she experience and cope with the disease.

Feline asthma is a condition very similar to the respiratory disease affecting humans. It is causing, among other things, a constriction of the bronchial airways, an increase of mucus production and damage to, or erosion of, the epithelial membrane. The symptoms occur intermittently and do, in most cases, evolve during a long period of time. The aim of this essay was to investigate whether there are any nursing routines for feline patients with asthma in animal clinics and animal hospitals in Sweden, and if so, to compare similarities and differences. A literary study of research material also led to a suggestion of how to form a nursing routine for this type of patient.

Ten colic horses and seven healthy horses were included in a prospective study aiming to evaluate acid-base disturbances in colic horses. Traditional acid-base methods and measurements of lactate were used to study prognosis and changes before, during and after surgery. Strong ion gap, anion gap and base excess were compared to evaluate their ability to predict lactate. Of the ten colic horses that went through surgery, seven survived, two were euthanised and one died. Significant differences in plasma lactate levels were seen between the group of survivals and non-survivals, p=0,000025. No differences were seen in pH and base excess between the two groups.

This essay analyses the European Food Safety Authority's (EFSA) legitimacy. Theories on input- and output legitimacy are used in the examination. After analysing stakeholder representation, forum for deliberation, transparency, independence, and information sharing, input legitimacy in EFSA is assessed to be high. Output legitimacy was examined through the authority's outcome effectiveness, which was divided into compliance and effectiveness. Since food safety is an immense area, two important parts were chosen for the analysis of output legitimacy; transmissible/bovine spongiform encephalopathy (TSE/BSE) and genetically modified organisms (GMO).

Background: Mastitis in dairy cattle is a common ailment worldwide. A cause of mastitis can be bacteria such as Escherichia coli. Mastitis is not a deadly ailment and sometimes the dairy cows show no symptoms but if certain virulence genes are present in the bacteria that cause the mastitis, the bacteria can be transmitted to humans and cause severe diseases. The potential presence of enterohemorrhagic Escherichia coli (EHEC) in particular would be a major concern for human health.Aim: The aim for this study was to analyze the presence of virulence genes known to be present in E.coli strains isolated from dairy cows with mastitis in Sweden.Method: A Qiagen BIO ROBOT EZ1 was used to purify DNA from 90 bacterial cultures. A panel of virulence genes were amplified and biotinylated from the purified DNA by PCR and an E.coli based DNA microarray was used to detect presumed virulence genes in E.coli.Result: There were no samples that had all the genes traditionally used to classify E.coli as EHEC or potential EHEC.

Background: Cystic fibrosis (CF) is the most common hereditary disease that leads to an early death. Earlier CF was considered as a childhood disease but today the expected age is 50 years. CF affects all the epithelial cells in the body which makes mucus and other body fluids more viscous than normal. The thick mucus leads to among other things respiratory problems and infections of the lungs. Today there is no cure, only medicines that controls the symptoms.Aim: To describe parents experiences living with a child with CF.

Leber´s hereditary optic neuropathy (LHON), a disease affecting vision, is caused by several point mutations in mitochondrial DNA. Mutations leading to a defect NADH ubiquinone oxidoreductase protein will affect the respiratory chain and cause a disturbed ATP production. It is still unknown why this defect leads to the degeneration of retinal ganglion cells and cells in the opticus nerve as well as demyelination of axons in these areas. Analysis of mitochondrial DNA is an important tool in the diagnosis of the disease. At the present time analysis is based on cleavage by restriction enzymes, which only detects two of the most frequent mutations: m.3460G>A and m.11778G>A.

In the year 1783 a fissure eruption from Laki in the southern part of Iceland produced a large amount of volcanic gases during a period of eight months. The volcanic gases and aerosols spread across the northern hem sphere due to ideal weather conditions and had a substantial impact on the environment, human health and also climate effects. This, so called dry fog, caused severe health problems, which led to death casualties, killing animals, crops and other vegetation. The consequence was a widespread famine which was aggravated by a hard and long winter in 1783/84. Analysis shows that the Icelandic volcanoes are the primary risk to produce dry fog that can affect Sweden.

Denna rapport beskriver de behov vilka ligger tillgrund för att skapa en IT-säkerhetsdatabas. Rapporten tar även upp olika IT-säkerhetsproblem och för en diskussion om vad som är faran med dessa problem. Diskussionen förs inom två områden rörande IT-säkerhet.Det ena området är om IT-säkerhet i allmänhet och tar upp begreppet CIA. CIA står för de engelska begreppen Confidentiality, Integrity och Availability och på svenska, sekretess, integritet och tillgänglighet.Den andra delen handlar om lite mer specifika IT-säkerhetsproblem, som till exempel virus och sniffing.På uppdrag av FOI är en databas, vilken är avsedd för att lagra information rörande IT-säkerhet, skapad. Rapporten tar upp en del grundläggande databasteori och beskriver designen och implementationen av databasen.

Japansk encefalit (JE) orsakas av Japansk encefalit virus och är en allvarlig myggburen sjukdom med upp till 50 000 humanfall per år. Kliniskt utvecklad sjukdom har en letalitet på runt 25 % och många som överlever drabbas av svåra neurologiska skador. Sjukdomen har en stor geografisk utbredning i Asien och sjukdomsfall ses främst på landsbygden. Viruset vidmakthålls i en zoonotisk cykel mellan mygg (främst Culex tritaeniorhynchus), vadande fåglar och tamsvin. Människor är accidentella värdar.

Children are a group of patients that require special knowledge from the anesthesia staff, both general and specific. Theoretical knowledge and practical experience is necessary to adequately care for the child as it anatomically, physiologically and mentally may differ from adult patients.A growing problem in the pediatric population is overweight and obesity. From a stagnation in the early 2000s the prevalence of overweight and obesity among children in the world are again increasing.The purpose of this study was to examine peri-operative care of overweight and obese children.Systematic searches were done in PubMed, CINAHL and Scopus. After the quality audit according to SBU, twelve articles were included for analysis.Results suggest that overweight and obese children have a higher risk of respiratory peri-operative complications. The risk of hospital admission after outpatient surgery was also greater in this group as well as higher costs for health care.Overweight and obese children are complex subjects with more frequent peri-operative risk factors than normal-weight children.