ABSTRACTMultiple sclerosis (MS) is the leading cause of adult disability inSweden. Hematopoietic stem cell transplantation (HSCT) can be used as a treatment option for patients with MS where the disease has an aggressive course. The treatment is preceded by high-dose chemotherapy which lowers the patient's immune system. It is therefore of interest to study infectious complications associated with HSCT in this patient.Aim: The purpose of this study was to identify infection problems in this population associated with HSCT, and to study differences in infectious complications between this group and patients diagnosed with lymphoma who have undergone the same treatment.Method: The study was a retrospective descriptive study with quantitative data. A medical record review was conducted.

Today there are no analgesic drugs approved to be used in goats. Drugs approved for use in other species are consequently being used without knowing the pharmacokinetics of these drugs in goats. The purpose of this study was to investigate the pharmacokinetics of the opioid butorphanol when given subcutaneously in goats. Subcutaneous administration might extend the half-life compared to intravenous or intramuscular administration. Eight clinically healthy, non-pregnant 6 months old female goats were used.

Glycogen is the storage form of glucose and is used by the skeletal musculature as energy both for intense and endurance events. The glycogen content in the horse?s middle gluteal muscle is more than 600 mmol/kg dry weight, and almost 400 mmol/kg dry weight in the human?s Vastus lateralis. In some special occasions, for example in competitions with several moments on following days, there is a need of a fast muscle glycogen resynthesis. The feed intake is of importance for the velocity of the resynthesis.

The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

An increasing number of peole are resisting the attraction of living around nature and opting instead to spend their lives in cities. Parks and green areas play an important part in our well-being and we become fascinated with the diversity of nature, in which we find a peacefulness that is unique. Amongst other things, being surrounded by nature can reduce high blood presure, lower the production of stress hormones and stimulate the mind and our ability to concentrate. It is therefore vitally important to entice city residents to parks and green areas to improve their physical and mental well-being. Previous research has shown that humans are seeking for certain characteristics in the parks and green areas which they are visiting.

Bakgrund: Antalet personer med diabetes i världen ökar ständigt. Dåliga levnadsvanor ökar risken för diabeteskomplikationer. Vården har ett ansvar att stärka patientens motivation och förmåga till egenvård. Syfte: Undersöka effekten av MI som behandlingsmetod för att hjälpa diabetespatienter till förbättrad egenvård.   Metod: Litteraturstudie. Artikelsökningar gjordes i de medicinska databaserna Pubmed, Cinahl och Scopus. I studien inkluderades 10 originalartiklar varav fem var av medelkvalitet och fem av hög kvalitet.

The genus Campylobacter is globally recognised as the leading bacterial cause of human foodborne gastroenteritis. Every year around 8000 Swedes are infected by Campylobacter. Most people are infected by thermotolerant Campylobacter species, commonly C. jejuni and C. coli.

Commonly held dietary guidelines discourage full-fat dairy consumption due its to high levels of saturated fatty acids, which are believed to aversely influence cardiovascular disease risk. Cardiovascular diseases cause more than one third of deaths in the developed world. With dairy often providing a large part of energy and nutrients in Western diets, research into its effects on human health is warranted. Several recent studies have suggested a protective role of dairy in the development of cardiometabolic disease. However, many of these have used imprecise methods of dietary assessment, based on questionnaires or interviews. Biomarkers are emerging as a means of evaluating diet in a more objective way. Currently used dairy fat biomarkers are minor fatty acid constituents of dairy that preferably are unique to dairy and not influenced by endogenous metabolism.

The aim of the project was to study the effect of oral administration of glucose and leucin on the resynthesis of muscle glycogen after intense exercise in Standardbred trotters. In a crossover design, 11 Standardbred trotters performed a standardized exercise test on two occasions in order to deplete muscle glycogen. The exercise test comprised a warm up period, 7 repeated bouts of exercise on an uphill 500 m slope, and a recovery period. After the exercise test horses received: 1) a solution of glucose and leucine via a nasogastric tube at 0 minutes and at 240 minutes post exercise and a single bout of glucose at 120 minutes post exercise or 2) water (placebo) at 0, 120 and 240 minutes. Blood samples for measurement of glucose and insulin concentrations were collected at rest, when the horses returned to the stables directly after exercise and throughout the 6 hour recovery phase. During the first recovery hour, samples were collected every 15 minutes and then every 30 minutes over the next 5 hours.

The Clinical Pathology Laboratory at SLU in Uppsala has analysed several blood samples from Dachshunds with severe anemia. As there are no previous studies on this topic, a study was performed to evaluate if Dachshunds are more prone to severe anemia compared with other breeds. In addition, a retrospective case study on Dachshunds with severe anemia was done to search for common risk factors or diseases. Hemoglobin values in samples from 1272 Dachshunds and 2269 German Shepherds analyzed from September 1994 to October 2007 were used to compare the frequency of severe anemia in Dachshund and German shepherd. The samples were identified by laboratory information system.

Bipolar disorder is a severe, affective illness which causes a person to alternate between episodes of pathologically elevated mood (mania) and depression ? in between these episodes the mood is normal. The length and intervals between the episodes are highly variable among the ill, as are the severity which is divided into two major subgroups: bipolar I and II. Bipolar I disorder is the most severe because of the patient?s inability to realize that he or she is currently in a manic phase, in the case of bipolar II disorder, the patient is aware that he or she has an abnormally raised mood, a state which is called hypomanic state instead of manic.

Joint injury (inflammation) or osteoarthritis is the most common cause of poor performance in the athletic horse. Osteoarthritis is the ensuing degeneration of joint cartilage due to inflammation/injury. The single most important factor in the inflammation cascade of joint injuries is interleukin-1 (IL-1). Until recently, these injuries have been treated with products like hyaluronic acid, cortisone and polysulphated glycosaminoglycans (PSGAG). Several studies have not been able to demonstrate a significant difference in the clinical improvement of joint injuries treated with hyaluronic acid or PSGAG and saline.

Bakgrund: Sambandet mellan hjärtkirurgi med extracorporeal cirkulation (ECC) och blodtransfusion visar risker så som ökad morbiditet och mortalitet. Detta kan orsaka lidande för patienten och leda till kostnader för samhället. Att hantera och administrera blodprodukter är en omvårdnadshandling som sjuksköterskan ansvarar för och detta ställer därför krav på dennes kompetens. Syftet: Syftet med denna studie var att studera intensivvårdssjuksköterskors kunskap om att ge blodtransfusion till patienter som genomgått hjärtkirurgi med ECC och om de ansåg sig ha kunskap om hur blodprodukter ska handhas och administreras på ett patientsäkert sätt, samt vilket behov av kompetensutveckling de ansåg sig ha. Metod: Studien som utfördes hade en kvantitativ design med deskriptiv ansats. Ett icke-slumpmässigt urval gjordes på en Thoraxintensivvårdsavdelningen vid ett universitetssjukhus i Mellansverige och 30 intensivvårdssjuksköterskor inkluderades. Resultat: Intensivvårdsjuksköterskorna hade en mycket låg till låg kunskap om de postoperativa komplikationer vid hjärtkirurgi med ECC som kan ha ett samband med blodtransfusion. Inget statistiskt signifikant samband kunde fastställas mellan intensivvårdsjuksköterskornas upplevda kunskap och faktiska kunskap om dessa postoperativa komplikationer.

In the western world, strokes are the third most common cause of death, hitting mainly the elderly population. Smoking, high blood pressure, diabetes and overweight are known risk factors for causing strokes. Previous studies show that an increased level of particulate matter, PM, is also related to an increased number of strokes. The population in this study consists of patients over 65 years old who live in Malmö. Levels of particulate matter were measured from October 2004 to April 2005 at a central outdoor monitoring site in Malmö.

Schmallenberg virus (SBV) is a novel arthropod-borne orthobunyavirus emerging in Europe in 2011 to 2012. Acute SBV infection causes diarrhoea, fever and reduced milk production in dairy cattle, but it is mainly the reproductive disorders (abortions, malformed foetuses and stillborn animals) in ruminants that have caused substantial economical losses. The prevalence of the virus outside of Europe is poorly investigated. SBV or SBV-like antibodies were detected in Mozambique in 2013, which raised interest for a similar study in Tanzania. In this study in Tanzania, blood samples were collected from 478 sheep and goats from 39 herds in 15 different villages in three districts, covering areas in the north, south and east of Tanzania. The epidemiology of the virus was investigated by tracing antibodies by ELISA and mapping of the virus by PCR was started.