Inventories of epiphytic lichens on Scots pine (Pinus sylvestris) were conducted at three sites in east central Sweden: along the newly build motorway (Road E4), along the old road (Road 600) and in a reference site, to compare the effects of traffic pollution on lichen diversities. At each site, species richness, lichen mean sensitivity value (MK), air quality index of the forest stand/sample plot (LKI) and nitrogen impact value (N) were calculated and it was tested if there was any difference between the sites. Twenty-four species were observed in total, and Parmeliopsis ambigua and Hypogymnia physodes were the most common species at all sites. At Road E4, fewer lichen species were observed than at the other sites and this might be due to the impact of air pollution. The species richness at Road 600 was similar to that of the reference site, which might be explained by the improved air quality.