Sahlgrenska Academy at University of Gothenburg Department of Internal Medicine and Clinical NutritionAbstractTitle: Can probiotics prevent atopic dermatitis in children with high risk?Author: Laura Pienihäkkinen and Max OlinSupervisor: Heléne Bertéus ForslundExaminer: Anna WinkvistProgramme: Dietician study programme, 180/240 ECTSType of paper: Examination paper, 15 hpDate: May 22, 2012Background Allergic disease is today common, this including atopic dermatitis, which is the most common inflammatory skin disease in the world. Heredity has been shown to play a major role in the development of this disease. It is therefore of high interest for both the individual and society to find effective methods for preventing atopic dermatitis in individuals with high risk profile. Probiotics are beneficial for the bacterial profile in the intestine and it is therefore of interest to study if they through their anti-inflammatory effect could prevent atopic dermatitis.Objective The aim was to study whether administration of probiotics to the mother and the child (directly or via breast milk) could prevent atopic dermatitis in children with high risk profile (at least one first degree relative with a diagnosed allergic disease).Search strategy A systematic literature search was done in databases PubMed and Scopus with search terms maternal, prenatal, probiotics, allergy, atopic dermatitis, prevention, and children.Selection criteria Randomized, controlled human studies and original articles in English were included.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Wounds on horses are common and sometimes difficult to treat. The wounds are often big and could be discovered too late for primary sutures, which have to be applied within 8 hours from time of accident, before bacteria has been able to infiltrate the surrounding tissue. The healing is often complicated by delayed healing and wound infection with consequences like phlegmone, fistulation and proud flesh formation.To avoid these consequences, the traditional practice has been to administer systemic antibiotics alone, systemic antibiotics in combination with topical antibiotics or topical antibiotics alone. Because few antibiotics can be used in horses, is it important to household with the few substances that are available. In veterinary medicine today, the increasing resistance amongst pathogenic microbes such as MRSA presents a growing problem.

AbstractThe research project Liberation, supported by the European Commission under the Environment and Sustainable Development Programme (Fifth RTD Framework Programme) aimed at developing a decision support system for ecological risk assessment of contaminated sites has proposed a tiered system including the triad method. This method integrates contaminant chemistry and bioavailability analyses with observed ecotoxicological effects in soil and groundwater. In this study the triad method was used to perform a risk characterization of a former surface treatment and metal industry, now used for the student union buildings of the Mälardalen University in Eskilstuna, Sweden. On the site, high concentrations of copper, zinc and nickel have been detected in the soil in a preliminary investigation of contaminant levels with XRF. For performing the risk assessment of the site according to the triad method, contaminant bioavailability, soil metal concentrations and soil toxicity were determined and an ecological assessment was carried out on four selected soil samples (one reference soil and three samples with low (B), medium (C) and high (D) contaminant levels). Contaminant bioavailability was measured through leaching (ISO TC 190/S7) and biouptake tests (DGT).

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Problems with wild boars are increasing in Sweden and some people argue that the wild boar population must decrease. This is mostly the opinion by farmers, while the hunters want more wild boars in Sweden. Today the population is the strongest in the south of Sweden and in the eastern parts of the country. However the population is growing stronger in other parts of Sweden as well. The population origins from these areas where they escaped from pens around 1970.

En operation framkallar en icke önskvärd fysiologisk reaktion hos patienten som benämns som kroppens stressvar. Stressvaret innebär att sympatiska nervsystemet aktiveras och att endokrina ändringar erhålls, bland annat minskning i sekretionen av TSH och insulin samt ökning i sekretionen av ACTH, kortisol och ADH. En viktig uppgift för en veterinär är att välja premedicineringsläkemedel innan en operation. Premedicineringsläkemedel har som främsta uppgifter att motverka det perioperativa stressvaret hos djuret, minska smärta och förstärka anestesimedlens effekt. Syftet med det här arbetet är att beskriva vad som händer i kroppen vid ett perioperativt stressvar och hur svaret kan motverkas med hjälp av premedicineringsläkemedel. Premedicineringsläkemedel väljs och kombineras från klasserna sedativa, analgetika och antikolinergika. De vanligaste använda sederande läkemedlen på svenska veterinärkliniker är dopaminantagonisten acepromazin och alfa2-agonisten medetomidin.

Malassezia otitis in dogs is a common cause of visit to a veterinary service. In otitis externa due to Malassezia overgrowth the dog has a higher number of yeast organisms and/or a local inflammatory response to the micro organism. Common clinical signs include pruritus, erythema, swelling and increased exudate production. Clinical symptoms and results from cytology of ear swabs from the ear canal is the mainstay for diagnosis. Any underlying condition has to be identified and corrected to avoid relapse. The treatment options for infective otitis externa are based upon topical administration of eardrops containing antibiotics, steroids and antifungals.

Since long, Sweden has had exceptionally low calf mortality compared to most other countries. The tradition has been to keep calves in individual pens. With increasing number of cows per herd there is now a larger interest in more efficient and economical group systems for housing calves. However it has been noticed that bigger herds have more health problems and higher calf mortality than smaller herds. The early signs of disease are harder to detect when the animals are not fed manually.

Primary ciliary dyskinesia (PCD) is an important but unusual cause of chronic respiratory disease of young dogs. The purpuse of this paper is to study and present internationally publicated papers on PCD in dogs and present three dogs, in which PCD was suspected. PCD has been reported in many dog breeds as well as in people. In dogs an humans, PCD is caused by inborn genetical defects which causes structural and functional abnormalities of cilia in the respiratory organs and other cilia in the body. Situs inversus occurs in about half the cases in people and probably in dogs as well. Respiratory signs usually predominate.

The management of water resources and waste water varies between the countries of the EU. For many years, a large part of the water in Europe has been contaminated by, among other things, insufficiently treated waste water and emissions from agriculture. The EU Water Framework Directive 2000/60/EC and the Urban Waste Water Directive 91/271/EEC are intended to harmonize fresh-water management and waste water management within the whole EU, aiming at safeguarding drinking water of good quality and a high quality of all water within the EU today and in the future. There are however some problems concerning the waste-water directive. One problem is the huge investment needed in Slovakia to fulfil the demands of the waste-water directive for waste-water management in larger communities (with more than 2000 inhabitants).

Peritonitis is a potentially life-threatening disease in horses. With no published work from Sweden, the objective of the study was to describe the occurrence and demographics of horses with peritonitis and to evaluate the outcome of treatment in terms of short-term survival during a ten-year period in a large clinic in Sweden. Data were examined in a retrospective manner in 69 horses diagnosed with and treated for peritonitis at Universitetsdjursjukhuset (UDS) in Uppsala between 2002-2012. Demographic data examined included age, breed, gender, history, duration of illness before arriving at UDS, reason for seeking veterinary care, initial clinical findings (general state of health, heart rate, respiratory rate, mucous membrane appearance, rectal temperature, abdominal sounds, rectal examination, nasogastric tube results), abdominocentesis results (abdominal fluid analysis including visual inspection, leukocytes and protein, cytology, bacterial culture and sensitivity pattern), complete blood count (CBC), Serum amyloid-A (SAA) upon presentation and a follow up, plasma fibrinogen, plasma protein and albumin, treatments, length of hospitalisation and outcome. All medical records with the diagnosis of peritonitis were extracted from the medical records system Trofast. All records with any other diagnosis code referring to trauma such as rectal tear or ruptured uterus, recent abdominal surgery, external trauma or rupture in the gastrointestinal tract were excluded. To be included in the study, the peritoneal fluid should contain more than 20.000 cells/?L, have a peritoneal protein value of >30 g/L; or have a significantly changed peritoneal fluid sample (orange with increased turbidity or worse) in cases where no data on cells or protein were available. The horses were divided into two groups; one where the peritonitis was deemed to have an idiopathic aetiology (primary peritonitis) and the other group where the peritonitis had a possible aetiology such as intestinal parasites, impaction etc.

Mastitis is an important disease in dairy cattle. It is very costly for the farmers and affects a large number of animals. Few studies have been made investigating the outcome of treatment for acute clinical mastitis under Swedish conditions, and it can be problematic to use the results in foreign studies since both the microbial flora as well as treatments may be different from the situation in Sweden. In this project we have investigated data on clinical mastitis and antibiotic treatment in the dairy herd at Jällaskolan, Uppsala based on recordings during the last ten years. The material includes both Swedish Red (SRB) and Swedish Holstein (SLB) breeds, which have been kept at the farm under similar conditions.

Genom ökad användning på träningskläder har antibakteriella behandlingar blivit allt vanligare för den allmänna konsumenten. Dessa plagg marknadsförs ofta som antibakte-riella eller luktfria, men det som inte framgår är vilka ämnen som används för att uppnå den luktfria effekten. Vanligt förekommande är de behandlingar som är baserade på oorganiska silversalter. Medan leverantörerna av dessa produkter argumenterar för, argumenterar många miljövetare och forskare på bakterier emot antibakteriella produk-ter då dessa kan ha en skadlig inverkan på vår miljö och hälsa. Rapporten syftar till att ge större kunskap kring miljö- och hälsopåverkan vid användandet av textiler behandlade med silversalter samt till att analysera konsumenters inställning till antibakteriella kläder samt deras användar- och tvättvanor för träningskläder.

The bacterium Lactobacillus reuteri, which natural habitat is in the intestine of mammals and birds, uses glycerol as a substrate for production of the antimicrobial compound reuterin. Glycerol has been shown to decrease the number of Escherichia coli in human feces and it is believed to be due to in situ production of reuterin. E. coli belongs to the large family of Enterobacteriaceae, naturally occurring in the intestine, and pathogenic strains of E. coli have been shown to be one of several bacteria causing diarrhoea in calves.