Brämhults Juice AB is one of Sweden?s leading producers of fresh fruit juices and drinks. The fruit drinks last 3 days once opened and when stored in a refrigerator kept at 7°C. Since factors that shorten the drinks? best before date are usually not noticed until the entire batch has expired, it would be advantageous to find a way to accelerate the drinks? natural deterioration, so that an unusal short best before date is noticed before more products are shipped off to the stores.

During the last years, producers and consumers have become increasingly aware of food safety. Foodborne illness and foodborne injury are in best case unpleasant but they can also be fatal. HACCP (Hazard Analysis Critical Control Point) is a preventative food safety system which is aimed to guarantee production of safe food free from pathogens, foreign materials and substances with potential to cause adverse health effect. In order to succeed in developing, installing, monitoring and verifying a HACCP system, there is a mix of hurdles for the company to overcome. While larger food companies meet a difficult challenge, small and medium size enterprises often feel that the hurdles are almost impossible to manage.

Foot rot is a severe claw disease which occurs among sheep and goats in most parts of the world, with outbreaks in hot and humid weather. It is a contagious bacterial infection caused by Dichelobacter nodosus in association with Fusobacterium necrophorum. Foot rot has a negative impact on both animal welfare and economy.This thesis presents how Australia, Nepal, Norway, Switzerland, Great Britain, Sweden and Uruguay handle foot rot. In the selection of countries the focus was on countries that have control- or eradication programs against foot rot. There are differences in the climate, animal husbandry practices, animal welfare and economic resources in the countries that are presented.

Canine otitis externa is a common reason for a visit to the vet. Predisposing factors often complicate treatment and may result in a chronic disease progression. All otic preparation for topical use in Fass Vet contain antibiotics, corticosteroids and a fungicide. Sixty cerumen samples from dogs with external otitis, sent to the Department of Bacteriology at SVA, were categorised according to their profile of isolated microorganisms. Staphylococcus intermedius alone or together with Malassezia pachydermatis were the most common organisms found. Fifteen percent of the samples contained M.

Background: Mastitis in dairy cattle is a common ailment worldwide. A cause of mastitis can be bacteria such as Escherichia coli. Mastitis is not a deadly ailment and sometimes the dairy cows show no symptoms but if certain virulence genes are present in the bacteria that cause the mastitis, the bacteria can be transmitted to humans and cause severe diseases. The potential presence of enterohemorrhagic Escherichia coli (EHEC) in particular would be a major concern for human health.Aim: The aim for this study was to analyze the presence of virulence genes known to be present in E.coli strains isolated from dairy cows with mastitis in Sweden.Method: A Qiagen BIO ROBOT EZ1 was used to purify DNA from 90 bacterial cultures. A panel of virulence genes were amplified and biotinylated from the purified DNA by PCR and an E.coli based DNA microarray was used to detect presumed virulence genes in E.coli.Result: There were no samples that had all the genes traditionally used to classify E.coli as EHEC or potential EHEC.

The purpose of this study was to examine whether urea could be an option as a nitrogen source for young cattle, the study was limited to risks related to feeding, growth and feed efficiency. Microbes in ruminants have the ability to convert nitrogen to protein, this has been highlighted by beef producers as they are in search of cheap protein feed resources. Urea can be a worthy source of nitrogen for growing young animals, because of its high nitrogen content and the low price. Studies have shown that the replacement of a portion of the total protein content by urea in diets with low content of crude protein, like corn silage, may improve animal growth and feed efficiency. Replacement of all the true protein with urea can result in reduced feed intake and microbial growth.

Safe retrieval of nutrients from human excreta to fertilize crops in order to gain adequate yields will increase food security and sustainability in agriculture areas with nutrient-poor fields. Safe and nutritious food will meet the dietary needs of the individual which promotes public health and the ability of an active life. Human excreta should be considered as a resource instead of being assumed as waste. Human faeces and urine is excellent fertilizers which may close the nutrient loop if utilized properly. However, sanitation of the excreta is required to prevent disease transmission by reducing the content of gastrointestinal pathogens such as bacteria, viruses and parasites. The objective of this study was to in small scale evaluate the sanitation in a combined liquid compost and urea treatment by monitor the inactivation of bacteria and viruses at the combination three start temperatures, 45, 40 and 35 °C, and three additions of urea, 0.5, 0.75 and 1 % and reference, 0 % urea addition.

Animals in zoos live in small enclosed areas. If a pathogen is introduced to the zoo, the animals are extremely vulnerable. The highest risk of introducing new pathogens occurs when new animals are introduced to the collection. Therefore it is essential that zoos have a protocol in place for disease prevention. This review has studied literature on the subject of disease prevention protocols when translocating animals between zoos.

ABSTRACT: Commercial swab transport systems are used for collection and transporting of fecal and other microbiological samples. This system must maintain viability and contribute to survival of microorganisms during transport to the laboratory. Four swab transport systems have been compared, eSwab, ?- Transwab and fecalSwab, all three with flocked swabs, and Copan Venturi Transystem with rayon swabs. The study followed the recommendations from the Clinical Laboratory Standard Institute; document M40-A for recovery of Samonella, Shigella, Yersinia and Neisseria gonorrhoeae after storage for 0, 6, 24, 48 and 72 h at room- (22-25°C) and refrigerated (2-8ºC) temperature.

An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined for potential errors. Fatal errors were pinpointed to the ligation reaction and the transformation required to generate and propagate the desired vector pETGH-(target)KO.

Oxochlorates are anions with a partially naturally occurrence in nature but are also spread by human activities, including the paper industry. These compounds are harmful to both nature and humans, which makes it necessary to find a good way for their degradation. There are two different kinds of bacteria that can use oxochlorates as electron acceptors in their metabolism, bacteria that break down perchlorate and bacteria that break down both perchlorate and chlorate. A bacterium that can break down chlorate under anaerobic conditions is Ideonella dechloratans which holds the genes for chlorite dismutase and chlorate reductase which are enzymes for the degradation of chlorate. Gene expression and enzyme activity of chlorite dismutase are induced under anaerobic conditions, which makes it interesting to find out how this regulation functions in order to better exploit these bacteria in biological wastewater treatment.

The aims of the study were to investigate the prevalence and antibiotic resistance of Campylobacter jejuni and Campylobacter coli in chicken and chicken products, and to investigate farmer awareness of antibiotic resistance development. The study was conducted in the Can Tho province in Vietnam during six weeks in October to November 2008. Ninety-six samples from raw chickens were collected from twelve market places and analysed for the presence of Campylobacter. A total of 96 cloacal swabs from 20 farms were obtained and analysed for the presence of Campylobacter. Farmers were asked to answer eight questions concerning the housing of the chickens, modes of handling sick animals as well as use and knowledge of antibiotics and resistance development to antibiotics.

The idea behind this project came from a feed study executed two years during the lambing season. Both years wool loss was experienced. Low temperature in combination with shearing was believed to cause the wool loss the first year, while protein deficiency was believed to be the cause the second year. In the literature many causes of wool loss are described; mechanical wear, telogen effluvium, bacterial dermatitis, external parasites, wool break, scrapie, genetic causes and nutritional deficiencies are some examples. The aim of this thesis was to study causes for wool loss and how common wool loss is in Swedish sheep herds.

Den ekologiska produktionen styrs av många olika lagar och regler som den konventionella produktionen inte behöver följa. Dessa regler kan ses som en välfärdsgaranti i den ekologiska slaktkycklingproduktionen då reglerna främst är skrivna för att kycklingarna ska kunna utföra sina naturliga beteenden i en passande miljö. Rastgårdar ska finnas till de ekologiska kycklingarna för att de ska få beta, få motion och få chansen att bygga upp sitt naturliga immunförsvar. I många fall ses rastgården som en positiv aspekt av den ekologiska produktionen, men det finns även problem relaterade till utevistelsen då exempelvis vilda djur kan lockas till slaktkycklingarna och på så vis stressa upp dem, smitta dem med parasiter och patogener eller äta upp dem. Det är vedertaget att den ekologiska produktionens dödlighet är högre än den konventionella produktionens på grund av rovdjuren och sjukdomarna och de ekologiska kycklingarna utsätts i många fall för fler smittorisker på grund av utevistelsen.

It is important to know what is normal in the wound healing process in order to determine what happens when a wound does not heal as expected. If the amount of bacteria in the wound gets too large the body will not be able to defend itself and it becomes an infection. Colonization and infection are common elements in wound management and it is often the veterinary nurse role to deal with wounds in a proper manner. Antibiotics are drugs that either kill or inhibit the multiplication of bacteria or fungi, and have been in veterinary medicine for approximately 60 years. Very few new antibiotics are introduced and increased resistance is a major problem that requires everyone to take responsibility to reduce the use of it.