The focus of this project was to characterize the occurrence of ESBL-­?producing Bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably EnteroBacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among Bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing Bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably EnteroBacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among Bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing Bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

The research in this paper aims to producing a home version machine thatcan dry and make biological waste into compact pellets. This product ismade primarily for those who want to improve the environment and reducethe energy consuming process of separating the biological leftovers fromother waste in the large processing plants and save money due to reducedpickups of waste. Since there is no commercially available product thatdoes what we want to achieve we had to start from the beginning with boththe design and the mechanical aspects. The result is a machine, that fits inour homes, and in an easy and inexpensive way reduces the amount ofbiological waste that needs to be collected by the public wastetransportation system. This is done without reducing any of the energy inthe waste and it can still be used to compost or by large-scale plants that usethe waste to make electricity.

Mulitresistent staphylococcus aureus (MRSA) is a Bacteria that is resistent against penicillin, this makes it difficulte to treat. MRSA causes woundinfections and other seriouse conditions. If MRSA establishes in an hospital environment it often spreads fast. Common ways of spreading MRSA is through direct or indirect contact. The nurse should work according to guidelines, apply hygienic routines and prevent the spread of infections.

Mastitis is the most common disease of dairy cows. Mastitis can be painful for the dairy cow and causes economical losses for the farmer. These losses are due to decreased milk production, more working hours for the farmer, treatment costs and possible costs due to culling of the cow. The udder inflammation, mastitis, is often caused by Bacteria infection. One of the most common Bacteria found to cause mastitis in Sweden is Staphylococcus aureus (S.

Ethanol for automotive purposes is an issue much debated and researchers disagree about the benefits of bioethanol. Converting energy-rich crops into ethanol suitable as motor fuel is a complex process and continuous development and improvements of processes and material used are important steps in the work of developing environmental friendly fuels. Grain is usually preserved by drying which consumes a lot of energy. Alternative storage of high-moisture grain in airtight storage systems reduce the energy required to produce bioethanol and studies have also shown that ethanol yields are increased more than 10% in ethanol fermentations of moist grain compared to dry. Airtight storage is often not perfectly airtight so biocontrol agents are necessary in order to preserve the grain.

Gastric and intestinal disorders are common and costly human health problems worldwide. Helicobacter pylori are a gram-negative, pathogen Bacteria and the most common cause of duodenal and gastric ulcer in the stomach the intestinal mucosa. Prolonged infection and colonization can lead to chronic gastritis and stomach cancer. H. pylori are present in the gastric and intestinal mucosa of half of the world population and a relation between infection and low socioeconomic status has been shown.

A sample of milk inoculated with S. aureus was treated with penicillin, and Antipen (a new commercial product containing penicillinase) was added. To another sample with S. aureus and penicillin, formic acid to pH 5 was added, and to yet another sample, a combination of formic acid and Antipen was added. Controls with no penicillin were treated in the same way.

The aim of this study was to see if an increased amount of gas could be seen in the small bowel of cats subjected to trauma and if measuring the amount of gas could be of any use in the clinical evaluation of the patient. When the peristaltic waves in the small bowel decreases due to neurological damage or chock the microorganisms of the gut grow in number and possibly produce more gas and toxins. When the cat is stressed it can swallow more air then usual, and the air that is in solution with the chyme in the lumen of the gut separates from the ingesta. When the bowel isn?t moving it becomes easier for Bacteria and toxins to penetrate the wall of the gut, and then get access to the bloodstream.

The complexity of the mast cell is becoming more prominent. From being the cell recognized just to contribute to allergic inflammation it is now also associated with wound healing, protection against tumor growth and in recognition and defense against a wide range of pathogens. The location of the mast cell in the barrier between the external and internal environment of the body enables the cell to quickly respond to invading Bacteria, virus and parasites. Diarrhea due to Escherichia coli infection, listeriosis, toxoplasmosis, Newcastle disease and bovine respiratory syncytial virus are examples of mast cell activating diseases that can affect animals in, as well as outside, the agricultural sector. Additionally, ticks and tick-borne diseases are of concern.

Hammarby Sjöstad is a new district of southern Stockholm built with focus on reduced environmental impact by recirculation of materials and sustainability. The environmental goals aim to cut the water consumption by half and a separate storm water treatment. Thus, the wastewater will be more concentrated and will originate from the households only. The Sjöstad project includes the idea of a local treatment plant for the household wastewater. To evaluate this possibility, Sjöstadsverket, an experimental treatment plant was constructed.

The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli BacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

Respiratory infections are one of the major causes of disease in sheep throughout the world. In the beginning of 2008 the Swedish National Veterinary Institute and the Swedish Animal Health Service together started a project about respiratory infections in Swedish sheep. This study is an initial part of that project and the aim of this study was to investigate different sampling possibilities with focus on nasal swabs. This study is composed partly of a literature review on causative agents of respiratory infections in sheep and existing sampling methods, and partly of an experimental study comprising 56 sheep. These sheep were sampled with both an ordinary nasal swab and a guarded nasal swab. Before the start of this study there was to our knowledge no described method for sampling sheep with a guarded swab.

The study evaluated the Mutant Prevention Concentration (MPC) of Pradofloxacin on three Escherichia coli (E.coli) strains, 2 wildtypes and one first-step gyrA resistant mutant. We also measured the value of AUC (Under the Concentration)/MPC that prevents growth of resistant mutants. It is of importance to reach a concentration above MPC that prevent E.coli from developing resistance against the antibiotic.We used an in vitro kinetic model where we added Bacteria? and antibiotic. The culture flask was attached to a pump with an adjustable pump-speed.

Good feeding practices are a basic aspect of good calf health. During the calf's first few months the calf is fed with purchased milk replacer or raw milk straight from the cow. On all farms, it will be a surplus of milk that may not be delivered to the dairy for various reasons. Milk that may not be supplied to the dairy includes colostrum and milk with unnormal properties, including milk from cows suffering from mastitis. This study investigated the possibility of improving the milk by pasteurization in order to secure its use as feed for calves and thereby obtain an improved calf health.

The purpose of this literature study was to examine what kind of composition fermented liquidfeed can have, what a desired composition is and possible advantages and disadvantages onhealth and growth performance of the pig. A well fermented liquid feed is characterized by a lowpH (.