Livsformerna på jorden delas systematiskt in i de tre domänerna bakterier, arkéer och eukaryoter. Arkéer är de mikroorganismer som lever i extrema miljöer såsom hetvattenkällor, sjöar med hög salthalt och i miljöer med extrema pH-värden. De kan existera i miljöer där inga andra organismer överlever men förekommer även rikligt överallt runtomkring oss, exempelvis i människans mage och som normalflora i munnen.Vissa bakterier och arkéer har genen för enzymet ammoniak monooxygenas (AMO). Detta enzym spelar en viktig roll vid rening av avloppsvatten genom att oxidera ammonium till nitrit.Syftet med detta examensarbete har varit att detektera arkéer i prover av aktivt slam vilket gjordes genom att optimera en Polymerase Chain Reaction (PCR) baserad metod. Först pelleterades slamproverna via centrifugering för att kunna preparera DNA.

Mjölksyrabakterier tillhörande släkten Lactobacillus och Bifidobacterium har nyligen upptäckts hos bin och i honungen de producerar och innefattar 13 nya arter[1]. Forskarna arbetar med att ta fram nya snabba och mer pålitliga identifierings metoder för att karakterisera dessa bakterier. I detta projekt undersöktes möjligheten att identifiera dessa bakterier med en ny metod som heter denaturing high performance liquid chromatography (DHPLC). Metoden bygger på separation av PCR (polymerase chain reaction) amplifierade 16S rDNA fragment i DHPLC [8]. Vid identifiering av bakterier amplifierades olika variabla regioner från 16S rRNA genen, som påvisade efter sekvensering störst genetisk variation mellan dessa bakterier [1]. Separationen utfördes med ion-pair reverse-phase high presure liquid chromatoghaphy (IP RP HPLC) med delvis denaturering av DNA molekylen.

Livsformerna på jorden delas systematiskt in i de tre domänerna bakterier, arkéer och eukaryoter. Arkéer är de mikroorganismer som lever i extrema miljöer såsom hetvattenkällor, sjöar med hög salthalt och i miljöer med extrema pH-värden. De kan existera i miljöer där inga andra organismer överlever men förekommer även rikligt överallt runtomkring oss, exempelvis i människans mage och som normalflora i munnen.Vissa bakterier och arkéer har genen för enzymet ammoniak monooxygenas (AMO). Detta enzym spelar en viktig roll vid rening av avloppsvatten genom att oxidera ammonium till nitrit.Syftet med detta examensarbete har varit att detektera arkéer i prover av aktivt slam vilket gjordes genom att optimera en Polymerase Chain Reaction (PCR) baserad metod. Först pelleterades slamproverna via centrifugering för att kunna preparera DNA.

Local participation in nature conservation has gained more attention over the last decades due to international conventions as well as local and national initiatives. This development can be seen as a reaction to a more expert oriented nature conservation characterized by centralized control and strict preservation of so called "original" nature. The overall objective of this thesis is to contribute to research on new forms of conservation where democracy and local participation is included. The case study focuses on the process of producing a pre-study for a suggested national park in southern Jämtland in the Swedish mountain region, and aims to investigate to what extent local participation was included. The pre-study was led by the Swedish Environmental Protection Agency (SEPA) and the County Administrative Board (CAB) in Jämtland.

Companies and special interest organizations pays multiple millions each year in order to reach out with messages about that their products are biggest, best and most beautiful. The publicity happens among thing like TV, in brochures, on the Internet andin newspapers etc. places. This means that consumer permanently becomes affected of publicity, and is thereby in an exposed situation. Around the EU, the border of what to consider undue or not, have been in different levels.

The problems around and the planning of wood flow is some of the most complex issues in the forest-sector. The experience and overview of the single employees is crucial for the possibility of correct tactical and economical decision-making. The increasing demand of savings and shorter reaction-times with greater flexibility in the wood-supply-chain, makes solutions and aids for improved effectiveness in the work and processes according to wood-flow urgent. Holmen Skog is starting to develop a new forest-road-management-system. The system will facilitate the work according to construction, improvements and updating the standard of roads.

Background: Hepatitis E (HEV) is a small, non-enveloped virus that belongs to the viral genus Hepevirus. HEV is a positive sense single-stranded RNA virus and there is insufficient information regarding its replication. This is mainly because the virus has low capacity to grow in normally used cell cultures. Many specific strand assay detection studies have been done in order to understand more about HEV replication. Unfortunately, these assays have the disadvantage of giving false positive results.Aim: The aim of this project was to improve the positive strand assay to increase specificity and eliminate false positivity which is due to high sensitivity of the polymerase chain reaction (PCR).

The most common form of hair loss in young and old people is androgenic alopecia. Hereditary hair loss affects both men and women. Hereditary hair loss in some women begins at the age of 30, but as a rule hair loss begins in post-menopausal women. In women the hair thins out in an oval portion of the front part of the scalp. In men, hair loss can start as early as at the age of 20.

The aim of the study was to examine individual staff experience and management of threats and violence in the acute psychiatric department for children and teenagers in Uppsala. Previous research shows that the experience of threats and/or violence is individual, and that it is difficult to distinguish between these concepts. Research shows that it is particularly vulnerable to work in psychiatry when mental illness is a cause when threats and violence occurs. Threats and violence can result in different consequences for the individual. The study is based on qualitative interviews and the material was collected in the autumn of 2012 at Akademiska sjukhuset.

AbstractIntroductionWedged shoe insoles are used as a treatment for different problems in order to change the ground reaction force. They can secondary cause different loading patterns under the foot compared to normal. No previous study has, to the author?s knowledge, clinically evaluated the plantar foot pressure with different full-length wedges in normal persons.PurposeThe purpose was to evaluate the plantar pressure with different degrees of full-length wedges in normal persons.MethodTwenty persons with normal feet have participated in this single blind study. Wedges were produced clinically in four different shapes, 3° valgus, 0° flat, 3° varus och 6° varus.

The overarching purpose of the study was to examine the effects of infant massage and to put these effects incontext with mother ? child attachment. The intention was also to study whether these effects have any relevancefor social work. This was accomplished by studying earlier research, theory and laws but also through interviewswith mothers who have followed through a course in infant massage. The main questions of the study was: Afterthe completion of the course and after using the massage technique at home, does the mother experience that thebond with their child is influenced by the technique and are the mothers more apprehensive of their child?ssignals? Has the mothers observed any signals from their infants during the massage and if so what has beentheir reaction? Does the mothers experience that the massage has provided any physiological effects? Has thecompletion of the course provided the mothers with knowledge that?s had an effect in their ways of parenting? Inwhat way does the earlier research, reports and laws deal with the benefits and risks of a secure or insecureattachment, and can the quality of the attachment have any meaning in the social work? A qualitative approachwas chosen with respondent-interviews.

INLEDNINGIntensivvårdsmiljön är en komplex och avancerad miljö där svårt sjuka patienter vårdas. Det är även denna patientgrupp som har störst risk att drabbas av en vårdskada, både pga. sitt tillstånd och för att de utsätts för avancerad vård och behandling i en högteknologisk miljö. I Kompetensbeskrivningen för intensivvårdssjuksköterskor påpekas att då miljön blir mera teknisk och avancerad krävs aktivt arbete för att uppnå patientsäkerhet. Socialstyrelsens statistik avseende vårdskador inom den somatiska hälso- och sjukvården visar att vårdskador är vanligt förekommande.

Insomnia is getting more prevalent with age, both because of natural changes in the sleep pattern and because of increased morbidity. It can cause dysfunctioning and sedation during the day. Therefore it is often treated with hypnotics. It is possible though that hypnotics can cause daytime sleepiness as well, not to mention increased risk of falling and fractures because of sedation and prolonged reaction time. Benzodiazepines and benzodiazepine related drugs, the subjects of this study, are commonly used as hypnotics.

Denna uppsats syftar till att dels beskriva, dels med utvald ekonomisk teori analysera regleringen av den svenska formen av kasinoverksamhet. Av särskilt intresse är att diskutera orsaken till den svenska lagstiftningen som reglerar spelverksamhet samt lagstiftningens relevans för en spelverksamhet som är utsatt för utländsk konkurrens. För att besvara syftet har följande frågeställningar formulerats: - Hur kan regleringen av den svenska spelverksamheten förklaras utifrån ekonomisk teori? - Vad är motiven till den svenska lagstiftningen som reglerar spelverksamhet? - Hur är kasinoverksamheten i USA och Storbritannien utformad? - Hur kan en framtida reglering av spelmarknaden utformas? Regleringen av spelmarknaden kan dels förklaras utifrån välfärdsteori, dels utifrån politisk ekonomi i form av särintresseteorin. Välfärdsteorin förklarar regleringen av spelmarknaden genom att den dels kan ses som en marknad som ger upphov till negativa externa effekter i form av spelmissbruk, dels som ett naturligt monopol då det finns stordriftsfördelar i information och kontrollen av verksamheten.

In October 2009, a new decision was made concerning the administration of predators which resulted in a new wolf policy in Sweden. This meant that the wolf population now should be limited to 210 individuals by harvest. The new policy also recognized that the public acceptance of this species must increase and the genetic status improved. In the 17th of December a decision was taken by the Swedish parliament that it should become legal to hunt wolves in Sweden. The wolf hunt resulted in debate on a large scale amongst people, some thought of it as a good decision while others thought it was wrong.