The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

AbstractBackground: When an acute illness or trauma occurs, life becomes disorganised and shattered for the critically ill person and his/her family members. The family members are confronted with thoughts about life and death which can cause stress and anxiety. How the family members cope with the crisis is individual and depending on personality and earlier experiences. Aim: The purpose of this literary review was to illustrate the family members´ experiences when dealing with a critically ill family member. Method: A literary review was made based on fifteen qualitative and quantitative scientific articles and a literary book.

Diabetes mellitus is a metabolic disorder that brings great changes in every individual's way of living. The goal in diabetes treatment is that the individual will be able to live a normal life, with normal length. To achieve this there is need to avoid complications, both acute and long-term. To be able to do this this the patient need knowledge about the illness and how to adjust pharmacological treatment to diet and exercise. In this, patient education is an important part.

This paper aims at shedding light on the effects of a broken psychological contract in terms of job satisfaction. In a qualitative field study, the author researches seven newly recruites employees about their expectations when starting their jobs at the rapidly growing, Swedish company Klarna. Using Herzbergs two-factor model of hygiene and motivation factors as well as modern motivation theory, i.e. self-determination theory, the study answers questions as to what makes a candidate choose a company and what factors motivates as well as demotivates the recruit at the job, in the onboarding phase. The result is an attempt to describe what makes a recruitment process successful..

Introduction: In Sweden the definition of ambulance nursing is the examination and treatment of sick or injured people done by ambulance nurses during transport.  This means not only focus on the illness or injury but also the human being treated. The paramedic service must be based on the presumption that the medical needs of the patients are guaranteed, and that the expectations and demands of the public are satisfied. Competence and availability are basic terms for a well-functional paramedic service. There is a lot of pressure put on the people working with pre-hospital nursing. One mistake can lead to serious consequences for the patient.

Background: Flavan-3-ols are polyphenolic phytochemicals belonging to the flavonoids. Flavan-3-ols are present in many higher plants and are particularly concentrated in e.g. Camellia sinensis (tea) and Theobroma cacao (cacao) and have got attention due to their antioxidative capacity. Many studies have been able to associate flavan-3-ol-rich foods to improved endothelial function. The aim of the present work is to review the scientific literature and evaluate the evidence for positive effects of flavan-3-ols on endothelial dysfunction in humans. Method: Scientific articles describing intervention and observational studies about flavan-3-ols and endothelial dysfunction, were searched for on http://www.pubmed.org.

The importance of energy- and environmental issues has increased, and the work towards reducing carbon dioxide emissions plays a major part. The European Union has set up goals for the membership countries to work towards this to happen. The carbon dioxide emissions in Sweden have been reduced the last few years, but there is still a lot to do. About 30 per cent of Sweden?s carbon dioxide emissions generate from energy usage related to housing, this is why the housing sector is of interest to examine.

Involving prospective users in a development process is important. This makes it easier to make a more usable product. This thesis is about developing an intranet targeting a school and focus is aimed towards intranet usability.The reader is given knowledge into how to design and implement an intranet using persona. Design and develop by using persona is difficult when there is a group with large variety of knowledge, age and technology interest, so as in this case. Persona although always helps during the development phase because of the strong links to the personas that are created, which gives developers a constant reminder of the end users of the system.The thesis describes how information is collected and processed.

The purpose of this thesis was to examine how the build-up of customer relations can be characterized at the end of the industrial selling process and how companies engage their cross-functional selling teams into this relationship building effort. Through a case study of Omega, a multinational company engaged in the selling of products of an industrial character, it was revealed that the build-up and maintaining of the customer relationship is of acute importance, as it presents the company with a window of opportunities. Through its relationship with the customer the company can be able to sell other products, which are related to the original purchase made by the customer. For the company?s competitors the established relationship might also serve as a barrier when attempting to enter the market or increase market share.

The issue of honour related problems in schools are important social problems in Sweden. It?s not unusual that honour related problem has had serious consequences before any action has been inserted by society. Sometimes the efforts made by society have had no impact whatsoever due to them being inserted too late. We can conclude based on our literature and our interviews, the importance of prevention.

Among researchers it has been established since decades that humans and animals have an internal biological clock that controls certain physiological mechanisms. One example regarding humans is the impairment of lung function during the night, causing the phenomenon ?nocturnal asthma? among asthmatics. Only a few studies concerning respiratory circadian rhythm in horses have been done previously and they showed a daily variation in horses with a chronic or an acute respiratory disease. The technique used in those studies was the conventional technique, which is invasive and less sensitive than the technique used in this study.

Background: War-related amputations are represented by a unique group of patients because of the wounds complexity and scope of several serious injuries. To prevent and avoid care suffering and suffering of the patient, this study focused on the nurse's way to manage and identify the needs of this unique group of patients who have suffered from war-related limb loss. To undergo a traumatic amputation is a big change for the victim, and the nurse has an important role in responding to the patient and their needs Aim: To illuminate nursing care of patients with war-related limb loss. Method: The chosen method was a literature review. The data consisted of nine qualitative and three quantitative studies.

The study aimed to evaluate the information and training the stoma therapist at Uppsala University Hospital has given to patients who have recently undergone stoma surgery. The study was a descriptive cross-sectional study using qualitative and quantitative design. A total of 22 patients who completely or partially took care of their stomas and recently had undergone stoma surgery participated by answering a questionnaire. At discharge most of them experienced they were relatively well-informed but they wanted more information. Before the revisit they weren?t especially safe or comfortable.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Syftet med denna kvalitativa intervjustudie var att belysa vad sjuksköterskor på ambulansen respektive akutmottagningen ansåg vara viktig information till patienter med akuta bröstsmärtor samt vad som var viktigt att tänka på vid informering av dem. Tio intervjuer med sjuksköterskor utfördes, fem på ambulansen samt fem på akutmottagningen. Resultatet har delats upp utifrån de olika arbetsplatserna. Resultatet visade att det fanns stora likheter i hur sjuksköterskorna svarade på frågorna som ställts. Det som ansågs viktigt att ge information om var vad som skedde och varför, det var även viktigt att informera om läkemedel och dess biverkningar och att med den information de gav, lugna patienterna.