En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

In southern Sweden, beech (Fagus silvatica) and spruce (Picea abies) are two species with similar demands on soil properties. With increased global warming and high acid deposition the future may bring larger areas were the two species are overlapping, competing for the same soil. The aim of this study was to compare spruce and beech concerning distribution, cultivation and soil properties. From available data comparisons were made between spruce and beech forests in Sweden. Soil analyses were made on samples from beech forest in southern Sweden, with and without natural regeneration of spruce.

Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.

Total mixed rations (TMR) and TMR complemented with individual grain feeding to dairy cows are a growing practice in Sweden. This is because the heard sizes of the farms are increasing. Some farmers and farm advisors in Sweden have great knowledge and practical experience of working with TMR. In the same time there are many advisors that do not know how to handle TMR and farms that have a group based feeding of their animals. In this MSc thesis, the knowledge of farmers that use TMR and advisors with lots of experience of TMR are gathered, with the aim to create a guide book for advisors that are about to start or just have started working with TMR.

The aim with this study was to investigate the effects a change of manufacturing process would have on the mechanical properties of drill bits made of a WC/Co composite used for stone cutting. The method used today is sintering, where the material is heated in a conventional sintering oven. The other method was microwave sintering, where the material is heated by radiation in the microwave region. Also an investigation of the manufacturing cost were made.The main difference between the two heating methods is that the conventional way is a rather slow process and the microwave method is very fast. The material is also heated homogeniously in the method with mirowaves, aposed to the case with the conventional sintering where the material is heated from the outside in.This makes the material harder and more wear resistent.

During the summer of 2005 a number of steer producers in Skåne, Sweden, found that their animals graded less favourable according to the EUROP carcass grading system when slaughtered, than ordinary. The aim with this project was to determine factors that affects the production result in steer production and to find the reason to the lower classification. The project was initiated by Anita Persson, LRF, Skåne and was preformed with supervision from Ingemar Olsson, SLU, and Jens Fjelkner, Skånesemin. Ten producers of dairy steers located in Skåne was chosen for this study. The producers were interviewed to collect information about their steer production with respect to their planning and results.

The quality of bread wheat is a large subject to study. The quality rests on two legs. The first one is the inheritance of specific characters, which can be influenced through plant breeding and type of cultivar. The other one is the environmental influences, such as crop rotation, nutrition, harvest and storage. The latter factors are possibly to affect through cultivation practices, while other factors are not possible to influence on for the grower, such as seasonal changes of weather and the soil content.

Hundreds of thousands of piglets are castrated without anesthesia in Sweden every year so that we may be guaranteed meat without boar odor. The smell appears while cooking meat of boar and is described as urine- and sweat-like. High levels of androstenone and skatole are considered the main factors behind boar odor. Skatole is a metabolite of the amino acid tryptophan, which is formed in the colon of all pigs. Androstenone is a steroid hormone that is released from the Leydig cells in the testes of boars.

It is possible to affect the feed costs in different ways. By steeping or fermentation the digestibility of the feed is improved for the animals with a natural process in the wet feeding system Steeping means that the feed is soaked some hours before feeding. This process activatesenzymes and improves the digestibility of for example phosphorus in the feed and make it more accessible to the animal. Wet feeding can be fermented or not. Fermentationmeans that the feed has been soaked for a certain length of time so that a biological process is started.

This thesis raises the problem with acid mine drainage around the closed mine Finngruvan.The purpose of the investigation was to compare metal content in surface water in the areaand its catchments between year 2010 and 2003, also to make calculation of specific runoffand metal load, furthermore determine the recipients in the area. Surface water samplingwhere preformed at the same place as 2003 and also at a few other places of interest. Adigital elevation file were used in GIS to calculate the area of the watersheds in the region,observations in the field where also made. The collected data were used to make comparisons,calculations and conclusions. The result showed that the surface water 2010 had somewhatlower metal concentrations.

This report describes a new foundation method and compares it with a traditional founding of concrete. This new method is called the Koljern technique and uses cellular glass which is called Foamglas. The Foamglas is compounded to founding elements by lightweight steel beams. Foamglas is an insulation material with high compressive strength, and is rot, insect, vermin and acid proof. The cellular glass is also fire proof and watertight, which makes it a very good construction material.The report contains a study of a number of cases for which the Koljern technique is compared with a foundation of concrete.

This bachelor thesis is intended to gather the knowledge and experimental data on the winter wheat crop production relevant for Swedish conditions, with focus on sowing time, row spacing and seed rate. This essay is a literature study with a minor component based on interviews with farmers producing winter wheat. The work is divided into a background part where basic knowledge of winter wheat physiology, development and environment interactions are described and one part where results of field trials studies are presented. Optimal date of sowing winter wheat depends on annual weather variations. Because it is difficult to forecast the weather the choice of sowing date should be based on weather statistics, production experience and previous results depending on sowing date.

The purpose of this report was to compare two different options for expanding of an existing pig farm. For the time being there are low prices within the agricultural market of pig production, and it is interesting to see if it is worth expanding or not. The two different options this report is based on are: expanding from a herd with 50 sows in breeding gilt production to a herd with 100 sows in breeding gilt production (option 1), or expanding to a herd with 140 sows in breeding gilt production (option 2). The number of pregnant gilts produced per year will stay the same for both alternatives; about 300 pregnant gilts per year. The rest of the breeding gilts will be sold at a weight of 25 kg. The pigs that are not sold as breeding gilts (e.g. castrates, and female pigs that are too small or that have disorders) will be kept on the farm as growing-finishing pigs until 120 kg of live weight, and then sold to the slaughter house. By using the existing buildings, even after the expanding, the investment costs, and thereby costs for housing, were kept as low as possible.

The tribo system ice-curling stone was investigated in order to understand the mechanisms behind the stones' behavior on the ice sheet. The problem with non-identical stones should also be addressed.The stone curls, that is, its sliding path deviates from a straight line to the right for a clock-wise rotation and to the left for a anti-clock-wise rotation. Several mechanisms to explain this behavior have been proposed over the years but none has been successful.By carrying out experiments at the local curling rink and studying silicon castings of ice- and stone-surfaces with scanning electron microscopy and vertical scanning interferometry, it has been decided that the curl is not due to dry friction, ice-debris or the difference in friction on the left and right side of the stone. The side force comes from the fact that the friction is higher at the back of the stone than at the front.The contact between stone and ice is never completely dry, nor in the hydrodynamic lubrication regime. It is probably a combination of hydrodynamic lubrication and a contribution from mechanical scratching of the ice.